288109

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 48125..48732 Replicon plasmid 4
Accession NZ_LR135261
Organism Enterococcus faecium isolate E4457

Toxin (Protein)


Gene name mazF Uniprot ID E3USU0
Locus tag EQB59_RS15210 Protein ID WP_002321032.1
Coordinates 48125..48475 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A829F3C0
Locus tag EQB59_RS15215 Protein ID WP_002287514.1
Coordinates 48469..48732 (-) Length 88 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EQB59_RS15180 43141..43695 - 555 WP_002323026.1 hypothetical protein -
EQB59_RS15185 43698..44540 - 843 WP_002323027.1 ParA family protein -
EQB59_RS15190 44860..45153 - 294 WP_002326890.1 hypothetical protein -
EQB59_RS15195 45369..45719 - 351 WP_002323028.1 hypothetical protein -
EQB59_RS15200 45712..47037 - 1326 WP_002319870.1 Y-family DNA polymerase -
EQB59_RS15205 47487..48101 + 615 WP_002318230.1 recombinase family protein -
EQB59_RS15210 48125..48475 - 351 WP_002321032.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
EQB59_RS15215 48469..48732 - 264 WP_002287514.1 hypothetical protein Antitoxin
EQB59_RS15220 49073..49252 - 180 WP_002305761.1 hypothetical protein -
EQB59_RS15230 49633..50340 - 708 WP_127837488.1 hypothetical protein -
EQB59_RS15235 50333..50770 - 438 WP_002296237.1 hypothetical protein -
EQB59_RS15240 50785..51036 - 252 WP_002296238.1 hypothetical protein -
EQB59_RS15245 51036..51242 - 207 WP_086328673.1 DNA helicase UvrA -
EQB59_RS15255 51683..51943 - 261 WP_002296240.1 hypothetical protein -
EQB59_RS15260 52309..52794 - 486 WP_002296241.1 single-stranded DNA-binding protein -
EQB59_RS15265 52797..53521 - 725 WP_164856672.1 nucleotidyl transferase AbiEii/AbiGii toxin family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..53521 53521


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(6-111)

Antitoxin

Antitoxin PemI-like

(4-87)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 13255.28 Da        Isoelectric Point: 8.0280

>T288109 WP_002321032.1 NZ_LR135261:c48475-48125 [Enterococcus faecium]
MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVVTVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLT
TIDYEARECIFLEKAHDSLVEELLMKVRTVFQKVSK

Download         Length: 351 bp

Antitoxin


Download         Length: 88 a.a.        Molecular weight: 10183.60 Da        Isoelectric Point: 4.6865

>AT288109 WP_002287514.1 NZ_LR135261:c48732-48469 [Enterococcus faecium]
MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQKRLIIEAANIENELTIEQLFENYTDERNHVTIQNLGE
AVGNEKW

Download         Length: 264 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829F5H8

Antitoxin

Source ID Structure
AlphaFold DB A0A829F3C0

References