Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Xre-MNT/HTH_26(antitoxin)
Location 24473..25581 Replicon plasmid 3
Accession NZ_LR135260
Organism Enterococcus faecium isolate E4457

Toxin (Protein)


Gene name MNTss Uniprot ID A0A2I4SCC6
Locus tag EQB59_RS14745 Protein ID WP_062810426.1
Coordinates 24712..25581 (+) Length 290 a.a.

Antitoxin (Protein)


Gene name Xress Uniprot ID B9DSK1
Locus tag EQB59_RS14740 Protein ID WP_002303393.1
Coordinates 24473..24697 (+) Length 75 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EQB59_RS14720 19474..20037 + 564 Protein_26 zinc ribbon domain-containing protein -
EQB59_RS14725 20480..21964 + 1485 WP_002294513.1 ABC-F type ribosomal protection protein Lsa(E) -
EQB59_RS14730 22018..22821 + 804 WP_002294514.1 lincosamide nucleotidyltransferase Lnu(B) -
EQB59_RS14735 23509..24330 + 822 Protein_29 recombinase zinc beta ribbon domain-containing protein -
EQB59_RS14740 24473..24697 + 225 WP_002303393.1 helix-turn-helix transcriptional regulator Antitoxin
EQB59_RS14745 24712..25581 + 870 WP_062810426.1 nucleotidyltransferase domain-containing protein Toxin
EQB59_RS14750 25562..26296 + 735 WP_000662263.1 class I SAM-dependent methyltransferase -
EQB59_RS14755 26329..27237 + 909 WP_001255866.1 aminoglycoside nucleotidyltransferase ANT(6)-Ia -
EQB59_RS14760 27234..27776 + 543 WP_000627290.1 streptothricin N-acetyltransferase Sat4 -
EQB59_RS14765 27869..28663 + 795 WP_001096887.1 aminoglycoside O-phosphotransferase APH(3')-IIIa -
EQB59_RS14770 29073..29156 + 84 Protein_36 peptide-binding protein -
EQB59_RS14775 29205..29288 + 84 WP_001814874.1 23S rRNA methyltransferase attenuation leader peptide -
EQB59_RS14780 29413..30150 + 738 WP_001038790.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B) -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Polymerase, nucleotidyl transferase domain

(12-111)

Antitoxin

Cro/C1-type helix-turn-helix domain

(4-57)


Sequences

Toxin        


Download         Length: 290 a.a.        Molecular weight: 32793.31 Da        Isoelectric Point: 4.6575

>T288108 WP_062810426.1 NZ_LR135260:24712-25581 [Enterococcus faecium]
MVGNVIQIVTEKLSSLPFIEGIVLGGSRARSTHTENSDIDIGIYYNSDSFDLTAINQIATELDDENRNNLVVPPGAWGDW
VNGGGWLVINGCHVDLILRDIKRVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
PTALKKSLMNFFIFEAEFSLMFVKANAGAEDKYYIAGHVFRIISCLNQVLFACNNAYCINEKKAIKLLETFEYKPEKYAE
RVNHIFEVLGLSLFECYDMTEKLYNEVNEIVSEINNFLNEESSDERKQI

Download         Length: 870 bp

Antitoxin


Download         Length: 75 a.a.        Molecular weight: 8351.65 Da        Isoelectric Point: 4.5357

>AT288108 WP_002303393.1 NZ_LR135260:24473-24697 [Enterococcus faecium]
MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLDELKAIADVLGVKFVQAFILPDGDEIKISNE

Download         Length: 225 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2I4SCC6

Antitoxin

Source ID Structure
AlphaFold DB A0A1M5SHM7

References