Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Xre-MNT/HTH_26(antitoxin)
Location 14182..15290 Replicon plasmid 3
Accession NZ_LR135260
Organism Enterococcus faecium isolate E4457

Toxin (Protein)


Gene name MNTss Uniprot ID A0A2H4HI44
Locus tag EQB59_RS14685 Protein ID WP_029170889.1
Coordinates 14421..15290 (+) Length 290 a.a.

Antitoxin (Protein)


Gene name Xress Uniprot ID -
Locus tag EQB59_RS14680 Protein ID WP_000205227.1
Coordinates 14182..14406 (+) Length 75 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EQB59_RS15650 9771..9941 + 171 WP_000713595.1 hypothetical protein -
EQB59_RS14655 9955..10557 + 603 WP_001062586.1 recombinase family protein -
EQB59_RS14660 10573..11250 + 678 Protein_14 DNA topoisomerase -
EQB59_RS14665 11762..12058 + 297 WP_002360685.1 hypothetical protein -
EQB59_RS14670 12059..12475 + 417 WP_000323438.1 recombinase -
EQB59_RS14675 12477..14039 + 1563 WP_000136908.1 recombinase family protein -
EQB59_RS14680 14182..14406 + 225 WP_000205227.1 helix-turn-helix transcriptional regulator Antitoxin
EQB59_RS14685 14421..15290 + 870 WP_029170889.1 nucleotidyltransferase domain-containing protein Toxin
EQB59_RS14690 15271..16005 + 735 WP_000662263.1 class I SAM-dependent methyltransferase -
EQB59_RS14695 16038..16901 + 864 WP_002294505.1 aminoglycoside nucleotidyltransferase ANT(6)-Ia -
EQB59_RS14700 16945..17472 + 528 WP_002294507.1 adenine phosphoribosyltransferase -
EQB59_RS14705 17604..18413 + 810 WP_002294509.1 ANT(9) family aminoglycoside nucleotidyltransferase Spw -
EQB59_RS14710 18631..18882 + 252 WP_002294510.1 hypothetical protein -
EQB59_RS14715 18933..19391 - 459 WP_002325145.1 hypothetical protein -
EQB59_RS14720 19474..20037 + 564 Protein_26 zinc ribbon domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Polymerase, nucleotidyl transferase domain

(12-114)

Antitoxin

Cro/C1-type helix-turn-helix domain

(4-57)


Sequences

Toxin        


Download         Length: 290 a.a.        Molecular weight: 32820.25 Da        Isoelectric Point: 4.9564

>T288107 WP_029170889.1 NZ_LR135260:14421-15290 [Enterococcus faecium]
MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIYYNSDSFDLTAINQIATELDDKNRNNLVVPPGAWGDW
INGGGWLFINGYHVDLILRDIKRVEQIMKDTEHGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAETY
PNALQKSLVNFFMFEAGFSLMFVKANSGTDDKYYIAGHVFRIVSCLNQVLFACNNAYCINEKKAIKLLETFEHKPEKYTE
KVNHIFEVLGISLFECYDMTEKLYNEVNEIVSEINNFLNEESSDERKQI

Download         Length: 870 bp

Antitoxin


Download         Length: 75 a.a.        Molecular weight: 8365.68 Da        Isoelectric Point: 4.5510

>AT288107 WP_000205227.1 NZ_LR135260:14182-14406 [Enterococcus faecium]
MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLDELKAIADVLGVKFVQAFILPDGEEIKISNE

Download         Length: 225 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2H4HI44

Antitoxin

Source ID Structure

References