Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Xre-MNT/HTH_26(antitoxin)
Location 133774..134882 Replicon plasmid 3
Accession NZ_LR135256
Organism Enterococcus faecium isolate E7098

Toxin (Protein)


Gene name MNTss Uniprot ID A0A0M4MHJ8
Locus tag EQB68_RS15730 Protein ID WP_002303392.1
Coordinates 133774..134643 (-) Length 290 a.a.

Antitoxin (Protein)


Gene name Xress Uniprot ID B9DSK1
Locus tag EQB68_RS15735 Protein ID WP_002303393.1
Coordinates 134658..134882 (-) Length 75 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EQB68_RS15700 129070..129321 - 252 Protein_155 GNAT family N-acetyltransferase -
EQB68_RS15705 129566..130861 + 1296 WP_002297218.1 ISL3-like element ISEfa11 family transposase -
EQB68_RS15710 130962..131870 + 909 WP_002324522.1 IS982-like element ISEfm1 family transposase -
EQB68_RS15715 131873..132121 - 249 WP_002347175.1 hypothetical protein -
EQB68_RS15720 132118..133026 - 909 WP_001255866.1 aminoglycoside nucleotidyltransferase ANT(6)-Ia -
EQB68_RS15725 133059..133793 - 735 WP_000662263.1 class I SAM-dependent methyltransferase -
EQB68_RS15730 133774..134643 - 870 WP_002303392.1 nucleotidyltransferase domain-containing protein Toxin
EQB68_RS15735 134658..134882 - 225 WP_002303393.1 helix-turn-helix transcriptional regulator Antitoxin
EQB68_RS15740 135025..135846 - 822 Protein_163 recombinase zinc beta ribbon domain-containing protein -
EQB68_RS15745 136534..137337 - 804 WP_002294514.1 lincosamide nucleotidyltransferase Lnu(B) -
EQB68_RS15750 137391..138326 - 936 Protein_165 ABC-F type ribosomal protection protein Lsa(E) -
EQB68_RS15755 138381..139061 - 681 WP_001015311.1 IS6-like element IS1216 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid VanHAX / erm(B) / aph(3')-III / ant(6)-Ia / lnu(B) / tet(L) - 1..155151 155151
- inside IScluster/Tn erm(B) / aph(3')-III / ant(6)-Ia / lnu(B) / tet(L) - 98927..148173 49246


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Polymerase, nucleotidyl transferase domain

(12-111)

Antitoxin

Cro/C1-type helix-turn-helix domain

(4-57)


Sequences

Toxin        


Download         Length: 290 a.a.        Molecular weight: 32807.38 Da        Isoelectric Point: 4.7269

>T288101 WP_002303392.1 NZ_LR135256:c134643-133774 [Enterococcus faecium]
MVGNVIQIVTEKLSSLPFIEGIVLGGSRARSTHTENSDIDIGIYYNSDSFDLTAINQIATELDDENRNNLVVPPGAWGDW
VNGGGWLVINGCHVDLILRDIKRVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
PTALKKSLMNFFIFEAEFSLMFVKANAGAEDKYYIAGHVFRIISCLNQVLFACNNAYCINEKKAIKLLETFEYKPEKYAE
RVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINNFLNEESSDERKQI

Download         Length: 870 bp

Antitoxin


Download         Length: 75 a.a.        Molecular weight: 8351.65 Da        Isoelectric Point: 4.5357

>AT288101 WP_002303393.1 NZ_LR135256:c134882-134658 [Enterococcus faecium]
MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLDELKAIADVLGVKFVQAFILPDGDEIKISNE

Download         Length: 225 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M4MHJ8

Antitoxin

Source ID Structure
AlphaFold DB A0A1M5SHM7

References