Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) omega-epsilon-zeta/zeta-epsilon
Location 125296..126433 Replicon plasmid 3
Accession NZ_LR135256
Organism Enterococcus faecium isolate E7098

Toxin (Protein)


Gene name zeta Uniprot ID Q54944
Locus tag EQB68_RS15675 Protein ID WP_001284311.1
Coordinates 125570..126433 (+) Length 288 a.a.

Antitoxin (Protein)


Gene name epsilon Uniprot ID R2XCR7
Locus tag EQB68_RS15670 Protein ID WP_000301765.1
Coordinates 125296..125568 (+) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EQB68_RS15645 121794..123287 + 1494 WP_000947691.1 primase C-terminal domain-containing protein -
EQB68_RS15650 123422..123718 + 297 WP_000053907.1 replication control protein PrgN -
EQB68_RS15655 123742..124701 - 960 WP_002287225.1 IS30 family transposase -
EQB68_RS15660 124721..124972 + 252 Protein_147 chromosome partitioning protein ParA -
EQB68_RS15665 125070..125279 + 210 WP_000527318.1 peptide-binding protein -
EQB68_RS15670 125296..125568 + 273 WP_000301765.1 antitoxin Antitoxin
EQB68_RS15675 125570..126433 + 864 WP_001284311.1 toxin zeta Toxin
EQB68_RS15680 126696..127433 - 738 WP_001038796.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B) -
EQB68_RS15685 127558..127641 - 84 WP_031929417.1 23S rRNA methyltransferase attenuator leader peptide ErmL -
EQB68_RS15690 127690..127773 - 84 Protein_153 peptide-binding protein -
EQB68_RS15695 128183..128977 - 795 WP_001096887.1 aminoglycoside O-phosphotransferase APH(3')-IIIa -
EQB68_RS15700 129070..129321 - 252 Protein_155 GNAT family N-acetyltransferase -
EQB68_RS15705 129566..130861 + 1296 WP_002297218.1 ISL3-like element ISEfa11 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid VanHAX / erm(B) / aph(3')-III / ant(6)-Ia / lnu(B) / tet(L) - 1..155151 155151
- inside IScluster/Tn erm(B) / aph(3')-III / ant(6)-Ia / lnu(B) / tet(L) - 98927..148173 49246


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Zeta toxin domain

(27-214)

Antitoxin

Bacterial epsilon antitoxin

(2-90)


Sequences

Toxin        


Download         Length: 288 a.a.        Molecular weight: 32404.02 Da        Isoelectric Point: 6.9964

>T288100 WP_001284311.1 NZ_LR135256:125570-126433 [Enterococcus faecium]
MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGSGKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELV
KLYEKDVVKHVTPYSNRMTEAIISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETPSISPKETLEKELNRKVSGKEIQ
PTLERIEQKMVLNKHQETPEFKAIQQKLESLQPPTPPIPKTPKLPGI

Download         Length: 864 bp

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 10721.25 Da        Isoelectric Point: 6.4676

>AT288100 WP_000301765.1 NZ_LR135256:125296-125568 [Enterococcus faecium]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEVNLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYS
KQVLNKEKVA

Download         Length: 273 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 1GVN
PDB 3Q8X

Antitoxin

Source ID Structure
PDB 3Q8X
PDB 1GVN
AlphaFold DB A0A829F0A3

References