288096

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) omega-epsilon-zeta/zeta-epsilon
Location 39127..40264 Replicon plasmid 5
Accession NZ_LR135247
Organism Enterococcus faecium isolate E6988

Toxin (Protein)


Gene name zeta Uniprot ID Q54944
Locus tag EQB64_RS16520 Protein ID WP_001284311.1
Coordinates 39401..40264 (+) Length 288 a.a.

Antitoxin (Protein)


Gene name epsilon Uniprot ID R2XCR7
Locus tag EQB64_RS16515 Protein ID WP_000301765.1
Coordinates 39127..39399 (+) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EQB64_RS16490 35625..37118 + 1494 WP_000947691.1 primase C-terminal domain-containing protein -
EQB64_RS16495 37253..37549 + 297 WP_000053907.1 replication control protein PrgN -
EQB64_RS16500 37573..38532 - 960 WP_002287225.1 IS30 family transposase -
EQB64_RS16505 38552..38803 + 252 Protein_54 chromosome partitioning protein ParA -
EQB64_RS16510 38901..39110 + 210 WP_000527318.1 peptide-binding protein -
EQB64_RS16515 39127..39399 + 273 WP_000301765.1 antitoxin Antitoxin
EQB64_RS16520 39401..40264 + 864 WP_001284311.1 toxin zeta Toxin
EQB64_RS16525 40527..41264 - 738 WP_001038796.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B) -
EQB64_RS16530 41389..41472 - 84 WP_031929417.1 23S rRNA methyltransferase attenuator leader peptide ErmL -
EQB64_RS16535 41521..41604 - 84 Protein_60 peptide-binding protein -
EQB64_RS16540 42014..42808 - 795 WP_001096887.1 aminoglycoside O-phosphotransferase APH(3')-IIIa -
EQB64_RS16545 42901..43152 - 252 Protein_62 GNAT family N-acetyltransferase -
EQB64_RS16550 43397..44692 + 1296 WP_002297218.1 ISL3-like element ISEfa11 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid VanHAX / erm(B) / aph(3')-III / ant(6)-Ia / lnu(B) - 1..51931 51931
- inside IScluster/Tn erm(B) / aph(3')-III / ant(6)-Ia / lnu(B) - 12387..51168 38781


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Zeta toxin domain

(27-214)

Antitoxin

Bacterial epsilon antitoxin

(2-90)


Sequences

Toxin        


Download         Length: 288 a.a.        Molecular weight: 32404.02 Da        Isoelectric Point: 6.9964

>T288096 WP_001284311.1 NZ_LR135247:39401-40264 [Enterococcus faecium]
MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGSGKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELV
KLYEKDVVKHVTPYSNRMTEAIISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETPSISPKETLEKELNRKVSGKEIQ
PTLERIEQKMVLNKHQETPEFKAIQQKLESLQPPTPPIPKTPKLPGI

Download         Length: 864 bp

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 10721.25 Da        Isoelectric Point: 6.4676

>AT288096 WP_000301765.1 NZ_LR135247:39127-39399 [Enterococcus faecium]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEVNLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYS
KQVLNKEKVA

Download         Length: 273 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 1GVN
PDB 3Q8X

Antitoxin

Source ID Structure
AlphaFold DB A0A829F0A3

References