Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 16924..17531 Replicon plasmid 2
Accession NZ_LR135244
Organism Enterococcus faecium isolate E6988

Toxin (Protein)


Gene name mazF Uniprot ID E3USU0
Locus tag EQB64_RS14220 Protein ID WP_002321032.1
Coordinates 17181..17531 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A829F3C0
Locus tag EQB64_RS14215 Protein ID WP_002287514.1
Coordinates 16924..17187 (+) Length 88 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EQB64_RS14170 12008..12907 + 900 WP_010733696.1 nucleotidyl transferase AbiEii/AbiGii toxin family protein -
EQB64_RS14175 12910..13407 + 498 WP_002326885.1 single-stranded DNA-binding protein -
EQB64_RS14180 13773..14033 + 261 WP_002296240.1 hypothetical protein -
EQB64_RS14185 14478..14684 + 207 WP_002325537.1 hypothetical protein -
EQB64_RS14190 14732..14935 + 204 WP_110300627.1 hypothetical protein -
EQB64_RS14195 14951..15388 + 438 WP_126341263.1 hypothetical protein -
EQB64_RS14200 15381..16088 + 708 WP_002332739.1 hypothetical protein -
EQB64_RS14210 16468..16698 + 231 WP_126341264.1 hypothetical protein -
EQB64_RS14215 16924..17187 + 264 WP_002287514.1 hypothetical protein Antitoxin
EQB64_RS14220 17181..17531 + 351 WP_002321032.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
EQB64_RS14225 17555..18169 - 615 WP_002318230.1 recombinase family protein -
EQB64_RS14230 18483..18905 + 423 WP_002288787.1 hypothetical protein -
EQB64_RS14235 18915..19118 + 204 WP_002299573.1 hypothetical protein -
EQB64_RS14240 19329..19919 + 591 WP_065305702.1 recombinase family protein -
EQB64_RS14245 20072..20686 + 615 WP_065305703.1 recombinase family protein -
EQB64_RS14250 20710..21879 + 1170 Protein_24 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid aac(6')-aph(2'') / aph(2'')-Ia - 1..216239 216239


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(6-111)

Antitoxin

Antitoxin PemI-like

(4-87)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 13255.28 Da        Isoelectric Point: 8.0280

>T288092 WP_002321032.1 NZ_LR135244:17181-17531 [Enterococcus faecium]
MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVVTVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLT
TIDYEARECIFLEKAHDSLVEELLMKVRTVFQKVSK

Download         Length: 351 bp

Antitoxin


Download         Length: 88 a.a.        Molecular weight: 10183.60 Da        Isoelectric Point: 4.6865

>AT288092 WP_002287514.1 NZ_LR135244:16924-17187 [Enterococcus faecium]
MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQKRLIIEAANIENELTIEQLFENYTDERNHVTIQNLGE
AVGNEKW

Download         Length: 264 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829F5H8

Antitoxin

Source ID Structure
AlphaFold DB A0A829F3C0

References