Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) omega-epsilon-zeta/zeta-epsilon
Location 20829..21966 Replicon plasmid 5
Accession NZ_LR135239
Organism Enterococcus faecium isolate E7067

Toxin (Protein)


Gene name zeta Uniprot ID Q54944
Locus tag EQB63_RS15865 Protein ID WP_001284311.1
Coordinates 20829..21692 (-) Length 288 a.a.

Antitoxin (Protein)


Gene name epsilon Uniprot ID R2XCR7
Locus tag EQB63_RS15870 Protein ID WP_000301765.1
Coordinates 21694..21966 (-) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EQB63_RS15835 16401..17696 - 1296 WP_002297218.1 ISL3-like element ISEfa11 family transposase -
EQB63_RS15840 17941..18192 + 252 Protein_21 GNAT family N-acetyltransferase -
EQB63_RS15845 18285..19079 + 795 WP_001096887.1 aminoglycoside O-phosphotransferase APH(3')-IIIa -
EQB63_RS15850 19489..19572 + 84 Protein_23 peptide-binding protein -
EQB63_RS15855 19621..19704 + 84 WP_031929417.1 23S rRNA methyltransferase attenuator leader peptide ErmL -
EQB63_RS15860 19829..20566 + 738 WP_001038796.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B) -
EQB63_RS15865 20829..21692 - 864 WP_001284311.1 toxin zeta Toxin
EQB63_RS15870 21694..21966 - 273 WP_000301765.1 antitoxin Antitoxin
EQB63_RS15875 21983..22192 - 210 WP_000527318.1 peptide-binding protein -
EQB63_RS15880 22290..22541 - 252 Protein_29 chromosome partitioning protein ParA -
EQB63_RS15885 22561..23520 + 960 WP_002287225.1 IS30 family transposase -
EQB63_RS15890 23544..23840 - 297 WP_000053907.1 replication control protein PrgN -
EQB63_RS15895 23975..25468 - 1494 WP_000947691.1 primase C-terminal domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid VanHAX / lnu(B) / ant(6)-Ia / aph(3')-III / erm(B) - 1..51960 51960
- inside IScluster/Tn VanHAX / lnu(B) / ant(6)-Ia / aph(3')-III / erm(B) - 4313..48735 44422


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Zeta toxin domain

(27-214)

Antitoxin

Bacterial epsilon antitoxin

(2-90)


Sequences

Toxin        


Download         Length: 288 a.a.        Molecular weight: 32404.02 Da        Isoelectric Point: 6.9964

>T288089 WP_001284311.1 NZ_LR135239:c21692-20829 [Enterococcus faecium]
MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGSGKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELV
KLYEKDVVKHVTPYSNRMTEAIISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETPSISPKETLEKELNRKVSGKEIQ
PTLERIEQKMVLNKHQETPEFKAIQQKLESLQPPTPPIPKTPKLPGI

Download         Length: 864 bp

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 10721.25 Da        Isoelectric Point: 6.4676

>AT288089 WP_000301765.1 NZ_LR135239:c21966-21694 [Enterococcus faecium]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEVNLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYS
KQVLNKEKVA

Download         Length: 273 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 1GVN
PDB 3Q8X

Antitoxin

Source ID Structure
PDB 3Q8X
PDB 1GVN
AlphaFold DB A0A829F0A3

References