288081

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Xre-MNT/HTH_26(antitoxin)
Location 37151..38259 Replicon plasmid 5
Accession NZ_LR135230
Organism Enterococcus faecium isolate E7025

Toxin (Protein)


Gene name MNTss Uniprot ID A0A0M4MHJ8
Locus tag EQB58_RS16210 Protein ID WP_002303392.1
Coordinates 37390..38259 (+) Length 290 a.a.

Antitoxin (Protein)


Gene name Xress Uniprot ID B9DSK1
Locus tag EQB58_RS16205 Protein ID WP_002303393.1
Coordinates 37151..37375 (+) Length 75 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EQB58_RS16185 32972..33652 + 681 WP_001015311.1 IS6-like element IS1216 family transposase -
EQB58_RS16190 33707..34642 + 936 Protein_44 ABC-F type ribosomal protection protein Lsa(E) -
EQB58_RS16195 34696..35499 + 804 WP_002294514.1 lincosamide nucleotidyltransferase Lnu(B) -
EQB58_RS16200 36187..37008 + 822 Protein_46 recombinase zinc beta ribbon domain-containing protein -
EQB58_RS16205 37151..37375 + 225 WP_002303393.1 helix-turn-helix transcriptional regulator Antitoxin
EQB58_RS16210 37390..38259 + 870 WP_002303392.1 nucleotidyltransferase domain-containing protein Toxin
EQB58_RS16215 38240..38974 + 735 WP_000662263.1 class I SAM-dependent methyltransferase -
EQB58_RS16220 39007..39915 + 909 WP_001255866.1 aminoglycoside nucleotidyltransferase ANT(6)-Ia -
EQB58_RS16225 39912..40160 + 249 WP_002347175.1 hypothetical protein -
EQB58_RS16230 40163..41071 - 909 WP_002324522.1 IS982-like element ISEfm1 family transposase -
EQB58_RS16235 41172..42467 - 1296 WP_002297218.1 ISL3-like element ISEfa11 family transposase -
EQB58_RS16240 42712..42963 + 252 Protein_54 GNAT family N-acetyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid erm(B) / VanHAX / lnu(B) / ant(6)-Ia / aph(3')-III - 1..44498 44498
- inside IScluster/Tn erm(B) / VanHAX / lnu(B) / ant(6)-Ia / aph(3')-III - 102..43850 43748


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Polymerase, nucleotidyl transferase domain

(12-111)

Antitoxin

Cro/C1-type helix-turn-helix domain

(4-57)


Sequences

Toxin        


Download         Length: 290 a.a.        Molecular weight: 32807.38 Da        Isoelectric Point: 4.7269

>T288081 WP_002303392.1 NZ_LR135230:37390-38259 [Enterococcus faecium]
MVGNVIQIVTEKLSSLPFIEGIVLGGSRARSTHTENSDIDIGIYYNSDSFDLTAINQIATELDDENRNNLVVPPGAWGDW
VNGGGWLVINGCHVDLILRDIKRVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
PTALKKSLMNFFIFEAEFSLMFVKANAGAEDKYYIAGHVFRIISCLNQVLFACNNAYCINEKKAIKLLETFEYKPEKYAE
RVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINNFLNEESSDERKQI

Download         Length: 870 bp

Antitoxin


Download         Length: 75 a.a.        Molecular weight: 8351.65 Da        Isoelectric Point: 4.5357

>AT288081 WP_002303393.1 NZ_LR135230:37151-37375 [Enterococcus faecium]
MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLDELKAIADVLGVKFVQAFILPDGDEIKISNE

Download         Length: 225 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M4MHJ8

Antitoxin

Source ID Structure
AlphaFold DB A0A1M5SHM7

References