Detailed information of TA system TA288076

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): omega-epsilon-zeta/zeta-epsilon
• Location: 23938..24562
• Replicon: plasmid 5
• Accession: NZ_LR135223
• Organism: Enterococcus faecium isolate E7040

Toxin (Protein)

• Gene name: zeta
• Uniprot ID: -
• Locus tag: EQB60_RS15975
• Protein ID: WP_127822567.1
• Coordinates: 24212..24562 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: epsilon
• Uniprot ID: R2XCR7
• Locus tag: EQB60_RS15970
• Protein ID: WP_000301765.1
• Coordinates: 23938..24210 (+)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EQB60_RS15945	20436..21929	+	1494	WP_000947691.1	primase C-terminal domain-containing protein	-
EQB60_RS15950	22064..22360	+	297	WP_000053907.1	replication control protein PrgN	-
EQB60_RS15955	22384..23343	-	960	WP_002287225.1	IS30 family transposase	-
EQB60_RS15960	23363..23614	+	252	Protein_33	chromosome partitioning protein ParA	-
EQB60_RS15965	23712..23921	+	210	WP_000527318.1	peptide-binding protein	-
EQB60_RS15970	23938..24210	+	273	WP_000301765.1	antitoxin	Antitoxin
EQB60_RS15975	24212..24562	+	351	WP_127822567.1	zeta toxin family protein	Toxin
EQB60_RS15980	24596..25276	-	681	WP_001015311.1	IS6-like element IS1216 family transposase	-
EQB60_RS15985	25608..26576	+	969	WP_001059542.1	D-lactate dehydrogenase VanH-A	-
EQB60_RS15990	26569..27600	+	1032	WP_001079845.1	D-alanine--(R)-lactate ligase VanA	-
EQB60_RS15995	27606..28214	+	609	WP_000402347.1	D-Ala-D-Ala dipeptidase VanX-A	-
EQB60_RS16000	28484..29164	-	681	WP_001015311.1	IS6-like element IS1216 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	VanHAX	-	1..38599	38599
	-	inside	IScluster/Tn	VanHAX	-	2042..29164	27122

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (27-112)

Antitoxin

• Bacterial epsilon antitoxin (2-90)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12959.56 Da
• Isoelectric Point: 5.1679

>T288076 WP_127822567.1 NZ_LR135223:24212-24562 [Enterococcus faecium]
MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGSGKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELV
KLYEKDVVKHVTPYSNRMTEAIISRLSDQGGSVAKF

Nucleotide

• Length: 351 bp

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10721.25 Da
• Isoelectric Point: 6.4676

>AT288076 WP_000301765.1 NZ_LR135223:23938-24210 [Enterococcus faecium]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEVNLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYS
KQVLNKEKVA

Nucleotide

• Length: 273 bp

Structures

Antitoxin

• AlphaFold DB: A0A829F0A3