Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) omega-epsilon-zeta/zeta-epsilon
Location 23938..24562 Replicon plasmid 5
Accession NZ_LR135223
Organism Enterococcus faecium isolate E7040

Toxin (Protein)


Gene name zeta Uniprot ID -
Locus tag EQB60_RS15975 Protein ID WP_127822567.1
Coordinates 24212..24562 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name epsilon Uniprot ID R2XCR7
Locus tag EQB60_RS15970 Protein ID WP_000301765.1
Coordinates 23938..24210 (+) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EQB60_RS15945 20436..21929 + 1494 WP_000947691.1 primase C-terminal domain-containing protein -
EQB60_RS15950 22064..22360 + 297 WP_000053907.1 replication control protein PrgN -
EQB60_RS15955 22384..23343 - 960 WP_002287225.1 IS30 family transposase -
EQB60_RS15960 23363..23614 + 252 Protein_33 chromosome partitioning protein ParA -
EQB60_RS15965 23712..23921 + 210 WP_000527318.1 peptide-binding protein -
EQB60_RS15970 23938..24210 + 273 WP_000301765.1 antitoxin Antitoxin
EQB60_RS15975 24212..24562 + 351 WP_127822567.1 zeta toxin family protein Toxin
EQB60_RS15980 24596..25276 - 681 WP_001015311.1 IS6-like element IS1216 family transposase -
EQB60_RS15985 25608..26576 + 969 WP_001059542.1 D-lactate dehydrogenase VanH-A -
EQB60_RS15990 26569..27600 + 1032 WP_001079845.1 D-alanine--(R)-lactate ligase VanA -
EQB60_RS15995 27606..28214 + 609 WP_000402347.1 D-Ala-D-Ala dipeptidase VanX-A -
EQB60_RS16000 28484..29164 - 681 WP_001015311.1 IS6-like element IS1216 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid VanHAX - 1..38599 38599
- inside IScluster/Tn VanHAX - 2042..29164 27122


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Zeta toxin domain

(27-112)

Antitoxin

Bacterial epsilon antitoxin

(2-90)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12959.56 Da        Isoelectric Point: 5.1679

>T288076 WP_127822567.1 NZ_LR135223:24212-24562 [Enterococcus faecium]
MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGSGKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELV
KLYEKDVVKHVTPYSNRMTEAIISRLSDQGGSVAKF

Download         Length: 351 bp

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 10721.25 Da        Isoelectric Point: 6.4676

>AT288076 WP_000301765.1 NZ_LR135223:23938-24210 [Enterococcus faecium]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEVNLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYS
KQVLNKEKVA

Download         Length: 273 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 3Q8X
PDB 1GVN
AlphaFold DB A0A829F0A3

References