Detailed information of TA system TA288066

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): omega-epsilon-zeta/zeta-epsilon
• Location: 95209..95878
• Replicon: plasmid 2
• Accession: NZ_LR135204
• Organism: Enterococcus faecium isolate E7171

Toxin (Protein)

• Gene name: zeta
• Uniprot ID: -
• Locus tag: EQB62_RS15015
• Protein ID: WP_010708525.1
• Coordinates: 95483..95878 (+)
• Length: 132 a.a.

Antitoxin (Protein)

• Gene name: epsilon
• Uniprot ID: Q9AL01
• Locus tag: EQB62_RS15010
• Protein ID: WP_002331065.1
• Coordinates: 95209..95481 (+)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EQB62_RS14970	90508..91416	+	909	WP_001255866.1	aminoglycoside nucleotidyltransferase ANT(6)-Ia	-
EQB62_RS14975	91413..91955	+	543	WP_000627290.1	streptothricin N-acetyltransferase Sat4	-
EQB62_RS14980	92048..92842	+	795	WP_001096887.1	aminoglycoside O-phosphotransferase APH(3')-IIIa	-
EQB62_RS14990	93118..93690	+	573	WP_001079938.1	HTH domain-containing protein	-
EQB62_RS15000	93988..94884	+	897	WP_002334978.1	ParA family protein	-
EQB62_RS15005	94983..95192	+	210	WP_000527318.1	peptide-binding protein	-
EQB62_RS15010	95209..95481	+	273	WP_002331065.1	antitoxin	Antitoxin
EQB62_RS15015	95483..95878	+	396	WP_010708525.1	zeta toxin family protein	Toxin
EQB62_RS15020	95906..96586	+	681	Protein_111	IS6 family transposase	-
EQB62_RS15025	96638..96784	+	147	Protein_112	Txe/YoeB family addiction module toxin	-
EQB62_RS15030	96861..97190	+	330	WP_002292678.1	AbrB family transcriptional regulator	-
EQB62_RS15035	97180..97467	+	288	WP_002300557.1	type II toxin-antitoxin system RelE/ParE family toxin	-
EQB62_RS15040	97753..98610	+	858	WP_002292680.1	AAA family ATPase	-
EQB62_RS15045	98611..99159	+	549	WP_002292681.1	hypothetical protein	-
EQB62_RS15050	99419..100147	+	729	WP_010726886.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	erm(B) / ant(6)-Ia / aph(3')-III / aac(6')-aph(2'')	-	1..231143	231143
	-	inside	IScluster/Tn	erm(B) / ant(6)-Ia / aph(3')-III	-	86512..101768	15256

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (28-118)

Antitoxin

• Bacterial epsilon antitoxin (2-90)

Sequences

Toxin

Protein

• Length: 132 a.a.
• Molecular weight: 14867.86 Da
• Isoelectric Point: 5.2505

>T288066 WP_010708525.1 NZ_LR135204:95483-95878 [Enterococcus faecium]
MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGSGKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELV
KLYEKDVVKHVTPYSNRMTEALISRLSDQGYNLVIEGFCCKVLNLLSNKVE

Nucleotide

• Length: 396 bp

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10733.17 Da
• Isoelectric Point: 4.4865

>AT288066 WP_002331065.1 NZ_LR135204:95209-95481 [Enterococcus faecium]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEVNLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYS
KQILNKEKVA

Nucleotide

• Length: 273 bp

Structures

Antitoxin

• AlphaFold DB: A0A828ZL55