Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 516681..517252 Replicon chromosome
Accession NZ_LR135191
Organism Enterococcus faecium isolate E4438

Toxin (Protein)


Gene name mazF Uniprot ID Q3Y2B8
Locus tag EL354_RS02605 Protein ID WP_002286801.1
Coordinates 516911..517252 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A828ZZN9
Locus tag EL354_RS02600 Protein ID WP_002323011.1
Coordinates 516681..516911 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EL354_RS02560 512047..513378 + 1332 WP_002330022.1 FAD-containing oxidoreductase -
EL354_RS02565 513400..514026 + 627 WP_002286816.1 cysteine hydrolase -
EL354_RS02570 514209..514790 + 582 WP_002286813.1 TetR/AcrR family transcriptional regulator -
EL354_RS02585 515276..515851 + 576 WP_002293673.1 SOS response-associated peptidase family protein -
EL354_RS02590 516056..516394 - 339 WP_002286804.1 hypothetical protein -
EL354_RS02600 516681..516911 + 231 WP_002323011.1 hypothetical protein Antitoxin
EL354_RS02605 516911..517252 + 342 WP_002286801.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
EL354_RS02610 517820..518982 + 1163 WP_086956687.1 IS3 family transposase -
EL354_RS02620 519237..519431 + 195 WP_002295146.1 hypothetical protein -
EL354_RS02625 519589..519871 + 283 Protein_490 transposase -
EL354_RS02635 520115..520945 - 831 WP_002286789.1 manganese catalase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13302.68 Da        Isoelectric Point: 9.9044

>T288055 WP_002286801.1 NZ_LR135191:516911-517252 [Enterococcus faecium]
VSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRKTMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQ
LKSLDFKERKLKKIENLPLQDMAKIDQIIQYMF

Download         Length: 342 bp

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8523.39 Da        Isoelectric Point: 3.9078

>AT288055 WP_002323011.1 NZ_LR135191:516681-516911 [Enterococcus faecium]
MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLVPKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL

Download         Length: 231 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FD66

Antitoxin

Source ID Structure
AlphaFold DB A0A828ZZN9

References