Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-couple_hipB
Location 1594388..1596032 Replicon chromosome
Accession NZ_LR134532
Organism Pasteurella multocida strain NCTC10323

Toxin (Protein)


Gene name hipA Uniprot ID V4NI19
Locus tag EL300_RS07445 Protein ID WP_005752891.1
Coordinates 1594725..1596032 (-) Length 436 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID V4NAB0
Locus tag EL300_RS07440 Protein ID WP_005752888.1
Coordinates 1594388..1594723 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EL300_RS07415 1590466..1591125 + 660 WP_005752318.1 bifunctional tRNA pseudouridine(32) synthase/23S rRNA pseudouridine(746) synthase RluA -
EL300_RS07420 1591189..1591758 + 570 WP_005752317.1 Der GTPase-activating protein YihI -
EL300_RS07425 1591764..1592192 + 429 WP_005752316.1 DUF2489 domain-containing protein -
EL300_RS07430 1592216..1593583 + 1368 WP_005752315.1 oxygen-independent coproporphyrinogen III oxidase -
EL300_RS07435 1593624..1594292 + 669 WP_005752314.1 NAD(P)H-dependent oxidoreductase -
EL300_RS07440 1594388..1594723 - 336 WP_005752888.1 helix-turn-helix domain-containing protein Antitoxin
EL300_RS07445 1594725..1596032 - 1308 WP_005752891.1 HipA domain-containing protein Toxin
EL300_RS07450 1596250..1597386 + 1137 WP_005752312.1 anhydro-N-acetylmuramic acid kinase -
EL300_RS07455 1597452..1600034 - 2583 WP_005752311.1 DNA mismatch repair protein MutS -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA-like, C-terminal

(158-375)

Antitoxin

Cro/C1-type helix-turn-helix domain

(39-94)


Sequences

Toxin        


Download         Length: 436 a.a.        Molecular weight: 50526.31 Da        Isoelectric Point: 4.9118

>T288023 WP_005752891.1 NZ_LR134532:c1596032-1594725 [Pasteurella multocida]
MEKLTLQIYLEQAWCDLAFLYFDSETYQLCNFEYLTDYVVQHYGKTGEHAVSLNYPVNVFIDDTTSLWSWLSDIAPAGAS
RRYWLEALDIKQESEEKQQFLLLKHAAISPIGNLRIKDAVPEQVEVRLFDLDKAKDRQSDFLEYVNQSGAMVGGATGAGG
EAPKLLLKRRENQVWIDNQQQGQDQDVSYLVKYPRGNYSPIDCDILRAEYHYYQELAQLGFDTIDTTRMRLEEGKRYPSL
WLPRFDIVQREGKTHRLAFESVYSLLQKQGGVLDHETTLRQLIERISTARLQFAQTDFVIEWVKRDLLNIAFGNSDNHGR
NMAFLRDEQRIWLAPIYDFAPMRADPEGIIRTTTWQTKGEYPQEFAGEYHFERIAEVLADLVEPTLLMQELRRLAGSLVD
LKSRLVQRNLPQSILDFPAIGFDYLPEKLKKWGLI

Download         Length: 1308 bp

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12520.60 Da        Isoelectric Point: 10.5259

>AT288023 WP_005752888.1 NZ_LR134532:c1594723-1594388 [Pasteurella multocida]
MQITRRNASTKGASVAEIDRKKVRNEIIQAMLMGELSQGEALRKLRIEMFGINQQHYIQLVKVSRQTLSNIENDKGNYSV
ETINKVFKPFGLELGLVPVSKALLKELFSTA

Download         Length: 336 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V4NI19

Antitoxin

Source ID Structure
AlphaFold DB V4NAB0

References