Detailed information of TA system TA028802

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 3341465..3342090
• Replicon: chromosome
• Accession: NC_020163
• Organism: Escherichia coli APEC O78

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: APECO78_RS16755
• Protein ID: WP_000911330.1
• Coordinates: 3341465..3341863 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: APECO78_RS16760
• Protein ID: WP_000450524.1
• Coordinates: 3341863..3342090 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
APECO78_RS16735	3337343..3337543	+	201	WP_000383836.1	YpfN family protein	-
APECO78_RS16740	3337653..3338351	-	699	WP_000679823.1	esterase	-
APECO78_RS16745	3338425..3340440	-	2016	WP_000829323.1	tRNA cytosine(34) acetyltransferase TmcA	-
APECO78_RS16750	3340455..3341318	-	864	WP_001267507.1	neutral zinc metallopeptidase	-
APECO78_RS16755	3341465..3341863	-	399	WP_000911330.1	tRNA(fMet)-specific endonuclease VapC	Toxin
APECO78_RS16760	3341863..3342090	-	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
APECO78_RS16765	3342244..3342957	-	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
APECO78_RS16775	3343170..3344204	-	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
APECO78_RS16780	3344221..3345099	-	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
APECO78_RS16785	3345245..3345817	+	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
APECO78_RS16790	3345817..3346287	+	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T28802 WP_000911330.1 NC_020163:c3341863-3341465 [Escherichia coli APEC O78]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T28802 NC_020163:c3341863-3341465 [Escherichia coli APEC O78]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT28802 WP_000450524.1 NC_020163:c3342090-3341863 [Escherichia coli APEC O78]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT28802 NC_020163:c3342090-3341863 [Escherichia coli APEC O78]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33