Detailed information of TA system TA287980

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 4706035..4706672
• Replicon: chromosome
• Accession: NZ_LR134501
• Organism: Nocardiopsis dassonvillei strain NCTC10488

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: D7B858
• Locus tag: EL646_RS20225
• Protein ID: WP_013155973.1
• Coordinates: 4706035..4706454 (-)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: D7B859
• Locus tag: EL646_RS20230
• Protein ID: WP_013155974.1
• Coordinates: 4706451..4706672 (-)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EL646_RS20210	4701562..4702431	+	870	WP_013155970.1	pantoate--beta-alanine ligase	-
EL646_RS20215	4702520..4705138	+	2619	WP_013155971.1	L-aspartate oxidase	-
EL646_RS20220	4705203..4705985	+	783	WP_013155972.1	type III pantothenate kinase	-
EL646_RS20225	4706035..4706454	-	420	WP_013155973.1	PIN domain nuclease	Toxin
EL646_RS20230	4706451..4706672	-	222	WP_013155974.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
EL646_RS20235	4706775..4707731	-	957	WP_013155975.1	cation diffusion facilitator family transporter	-
EL646_RS20240	4707828..4709432	+	1605	WP_081461785.1	bifunctional lysylphosphatidylglycerol synthetase/lysine--tRNA ligase LysX	-
EL646_RS20245	4710136..4710459	+	324	WP_041553490.1	LuxR family transcriptional regulator	-
EL646_RS20250	4710653..4711291	+	639	WP_013155978.1	amino-acid N-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-126)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (2-40)

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 15824.91 Da
• Isoelectric Point: 6.6034

>T287980 WP_013155973.1 NZ_LR134501:c4706454-4706035 [Nocardiopsis dassonvillei]
VNTVYLVDSSAWVEYLRKTGSETHVFVRELIERGVHLATTEPVAAEILSGAKSSTELQQLERLTDGLIQLSLDNRLDFHH
SAGIYREARSRGKTIRKLYDCLIAAVALRTDAVLVHCDRDFDHLAEVFPRLRVQRHDQD

Nucleotide

• Length: 420 bp

>T287980 NZ_LR134501:c4706454-4706035 [Nocardiopsis dassonvillei]
GTGAACACCGTCTATCTGGTGGACAGCTCCGCCTGGGTGGAGTACCTGCGCAAGACCGGTTCCGAGACCCACGTCTTCGT
CAGGGAACTCATCGAGCGCGGCGTCCACCTGGCCACGACCGAACCCGTCGCCGCCGAGATCCTGAGCGGGGCGAAGAGCT
CCACCGAGCTCCAGCAGCTGGAACGCCTCACCGACGGCCTGATCCAGCTGTCTCTGGACAACAGGCTCGACTTCCACCAT
TCGGCGGGCATCTACCGTGAGGCCCGCTCCAGGGGTAAGACGATCCGCAAGCTCTACGACTGCCTCATCGCGGCCGTCGC
CCTGCGCACCGACGCGGTCCTCGTGCACTGCGACCGCGACTTCGACCACCTCGCCGAGGTGTTCCCCCGGCTGCGCGTGC
AGCGGCACGACCAGGACTGA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8072.09 Da
• Isoelectric Point: 4.3094

>AT287980 WP_013155974.1 NZ_LR134501:c4706672-4706451 [Nocardiopsis dassonvillei]
MSRTNIDIDDELVTTAMERFQVSTKREAVDIALRRAVGTPLTKEFLLSLEGIGWEGDLEEMRRSGFEGGAPAS

Nucleotide

• Length: 222 bp

>AT287980 NZ_LR134501:c4706672-4706451 [Nocardiopsis dassonvillei]
ATGAGTCGCACAAACATTGACATCGACGACGAACTGGTCACTACCGCCATGGAACGCTTCCAGGTGAGCACCAAGCGCGA
AGCCGTGGACATCGCCCTTCGCCGGGCCGTGGGCACACCTCTCACCAAGGAGTTCCTCCTCAGCCTGGAGGGCATCGGCT
GGGAGGGCGACCTGGAAGAGATGCGCCGCTCCGGCTTCGAGGGCGGGGCGCCCGCCTCGTGA

Structures

Toxin

• AlphaFold DB: D7B858

Antitoxin

• AlphaFold DB: D7B859