Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1191840..1192458 Replicon chromosome
Accession NC_020163
Organism Escherichia coli APEC O78

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag APECO78_RS06015 Protein ID WP_001291435.1
Coordinates 1191840..1192058 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag APECO78_RS06020 Protein ID WP_000344800.1
Coordinates 1192084..1192458 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
APECO78_RS05985 1187129..1187701 + 573 WP_000779831.1 YbaY family lipoprotein -
APECO78_RS05990 1187732..1188043 - 312 WP_000409911.1 MGMT family protein -
APECO78_RS05995 1188422..1188775 + 354 Protein_1117 DUF1428 family protein -
APECO78_RS06000 1188817..1190367 - 1551 WP_001299455.1 cyclic-guanylate-specific phosphodiesterase PdeB -
APECO78_RS06005 1190531..1191001 - 471 WP_000136192.1 YlaC family protein -
APECO78_RS06010 1191117..1191668 - 552 WP_000102568.1 maltose O-acetyltransferase -
APECO78_RS06015 1191840..1192058 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
APECO78_RS06020 1192084..1192458 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
APECO78_RS06025 1193004..1196153 - 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
APECO78_RS06030 1196176..1197369 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T28791 WP_001291435.1 NC_020163:c1192058-1191840 [Escherichia coli APEC O78]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T28791 NC_020163:c1192058-1191840 [Escherichia coli APEC O78]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTTTGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT28791 WP_000344800.1 NC_020163:c1192458-1192084 [Escherichia coli APEC O78]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT28791 NC_020163:c1192458-1192084 [Escherichia coli APEC O78]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References