Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/RelB(antitoxin)
Location 1026457..1027093 Replicon chromosome
Accession NZ_LR134348
Organism Bifidobacterium breve strain NCTC11815

Toxin (Protein)


Gene name mazF Uniprot ID D4BP71
Locus tag EL174_RS04550 Protein ID WP_003829234.1
Coordinates 1026457..1026813 (-) Length 119 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID D6PAX1
Locus tag EL174_RS04555 Protein ID WP_012577921.1
Coordinates 1026800..1027093 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EL174_RS04520 1021648..1022559 + 912 WP_003829224.1 ABC transporter ATP-binding protein -
EL174_RS04525 1022756..1023382 + 627 WP_003829226.1 30S ribosomal protein S4 -
EL174_RS04530 1023719..1024501 - 783 WP_003829227.1 gamma-glutamyl-gamma-aminobutyrate hydrolase family protein -
EL174_RS04535 1024642..1025127 - 486 WP_003829229.1 cytidine deaminase -
EL174_RS04540 1025217..1025573 + 357 WP_003829232.1 SdpI family protein -
EL174_RS04545 1025678..1026280 + 603 WP_003829233.1 transglutaminase family protein -
EL174_RS04550 1026457..1026813 - 357 WP_003829234.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
EL174_RS04555 1026800..1027093 - 294 WP_012577921.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
EL174_RS04560 1027244..1027408 - 165 Protein_840 IS30 family transposase -
EL174_RS04565 1027508..1028182 + 675 WP_032738178.1 histidine phosphatase family protein -
EL174_RS04570 1028265..1028669 + 405 WP_003829238.1 DUF948 domain-containing protein -
EL174_RS04575 1028669..1028905 + 237 WP_003829239.1 hypothetical protein -
EL174_RS04580 1029001..1031679 + 2679 WP_003829240.1 alanine--tRNA ligase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-110)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(11-53)


Sequences

Toxin        


Download         Length: 119 a.a.        Molecular weight: 13316.23 Da        Isoelectric Point: 7.9984

>T287766 WP_003829234.1 NZ_LR134348:c1026813-1026457 [Bifidobacterium breve]
MMKTDPHQFEIWWVPFTFPDKPGKAKNRPSVILQWDDGTRIALVTKVTGNTWRDEPGYVVLRDWQDAGLSKPSAVRCSQL
LRLPSSLFLDDGPTGRLSAYDAGRVTWAINELYPGLLA

Download         Length: 357 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10866.31 Da        Isoelectric Point: 4.2896

>AT287766 WP_012577921.1 NZ_LR134348:c1027093-1026800 [Bifidobacterium breve]
MAIKEKTTISLDAQTKRDGIAILDAMGLNLSTFAEMSLRQLVRDGRLPFTPSVRPSFEKDNEGYPLFKANMDDPRIVTPQ
IRDGAVILPEGWDDDED

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0L7B6G7

Antitoxin

Source ID Structure
AlphaFold DB A0A1S2VTZ6

References