Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PemK-RelB
Location 967145..967700 Replicon chromosome
Accession NZ_LR134348
Organism Bifidobacterium breve strain NCTC11815

Toxin (Protein)


Gene name mazF Uniprot ID S2ZXD7
Locus tag EL174_RS04265 Protein ID WP_003829109.1
Coordinates 967145..967468 (-) Length 108 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S2ZDC4
Locus tag EL174_RS04270 Protein ID WP_003829112.1
Coordinates 967455..967700 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EL174_RS04230 962182..962470 - 289 Protein_778 IS256 family transposase -
EL174_RS04240 962941..964206 - 1266 WP_003829096.1 Fic family protein -
EL174_RS04250 964748..965113 - 366 WP_032738175.1 YccF domain-containing protein -
EL174_RS04255 965351..966220 + 870 WP_003829104.1 class I SAM-dependent methyltransferase -
EL174_RS04260 966314..966928 + 615 WP_003829107.1 cupin domain-containing protein -
EL174_RS04265 967145..967468 - 324 WP_003829109.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
EL174_RS04270 967455..967700 - 246 WP_003829112.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
EL174_RS04275 967874..969130 + 1257 WP_003829116.1 HipA domain-containing protein -
EL174_RS04280 969395..969586 + 192 WP_003829118.1 hypothetical protein -
EL174_RS04285 969770..971041 - 1272 WP_003829121.1 MFS transporter -
EL174_RS04290 971198..971860 - 663 WP_025262926.1 TetR/AcrR family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 956914..1005199 48285


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(2-103)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(5-56)


Sequences

Toxin        


Download         Length: 108 a.a.        Molecular weight: 11859.67 Da        Isoelectric Point: 5.0771

>T287765 WP_003829109.1 NZ_LR134348:c967468-967145 [Bifidobacterium breve]
MKRGEIWTLQADGYASKPRPVVIVQSDAVDRFDSVITCLLTSYDSSDIDTRVRLEPSPENGLNKVSYVMTDKIVTVDRKL
LGYQVGVVDDAAMANIGRQLMRVLGLL

Download         Length: 324 bp

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 9339.43 Da        Isoelectric Point: 4.5881

>AT287765 WP_003829112.1 NZ_LR134348:c967700-967455 [Bifidobacterium breve]
MSMQIATRVDDEQAALFKETTRQLGTTPADALRMFISAFNDYRGFPYEVRLPRNDVEPFASERDATEYASRLALRMSDET
R

Download         Length: 246 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A087B6Q7

Antitoxin

Source ID Structure
AlphaFold DB A0A087B6Q6

References