Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN_Sll0205-like-Phd
Location 3134083..3134687 Replicon chromosome
Accession NC_020089
Organism Mycobacterium tuberculosis 7199-99

Toxin (Protein)


Gene name vapC Uniprot ID P71623
Locus tag MT7199_RS14825 Protein ID WP_003414492.1
Coordinates 3134083..3134475 (-) Length 131 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A829CBY8
Locus tag MT7199_RS14830 Protein ID WP_003414495.1
Coordinates 3134472..3134687 (-) Length 72 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MT7199_RS14795 3129233..3130021 - 789 WP_003917092.1 CRISPR-associated endoribonuclease Cas6 -
MT7199_RS14800 3130355..3130900 - 546 WP_003913758.1 DUF1802 family protein -
MT7199_RS14805 3131172..3132056 - 885 WP_003414409.1 nucleotidyl transferase AbiEii/AbiGii toxin family protein -
MT7199_RS14810 3132059..3132946 - 888 WP_003414414.1 type IV toxin-antitoxin system AbiEi family antitoxin -
MT7199_RS14815 3133251..3133796 - 546 WP_003899500.1 DUF1802 family protein -
MT7199_RS14820 3133793..3134062 - 270 WP_003912856.1 DUF2277 family protein -
MT7199_RS14825 3134083..3134475 - 393 WP_003414492.1 type II toxin-antitoxin system VapC family toxin Toxin
MT7199_RS14830 3134472..3134687 - 216 WP_003414495.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
MT7199_RS14835 3134734..3135483 + 750 WP_003414497.1 enoyl-CoA hydratase -
MT7199_RS14840 3135562..3136644 - 1083 WP_003414499.1 ABC transporter ATP-binding protein -
MT7199_RS14845 3136637..3137947 - 1311 WP_003414501.1 ABC transporter substrate-binding protein -
MT7199_RS14850 3137950..3138777 - 828 WP_003414504.1 carbohydrate ABC transporter permease -
MT7199_RS14855 3138774..3139685 - 912 WP_003414505.1 sugar ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

VapC-like Sll0205 protein, PIN domain

(5-130)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(2-46)


Sequences

Toxin        


Download         Length: 131 a.a.        Molecular weight: 14610.74 Da        Isoelectric Point: 6.7594

>T28776 WP_003414492.1 NC_020089:c3134475-3134083 [Mycobacterium tuberculosis 7199-99]
MTTVLLDSHVAYWWSAEPQRLSMAASQAIEHADELAVAAISWFELAWLAEQERIQLAIPVLSWLQQLAEHVRTVGITPSV
AATAVALPSSFPGDPADRLIYATAIEHGWRLVTKDRRLRSHRHPRPVTVW

Download         Length: 393 bp

>T28776 NC_020089:c3134475-3134083 [Mycobacterium tuberculosis 7199-99]
ATGACGACGGTGCTGCTCGACTCGCATGTGGCCTACTGGTGGTCGGCCGAGCCGCAGCGTCTCAGCATGGCGGCGAGCCA
GGCCATCGAACACGCCGACGAGCTCGCCGTCGCCGCGATTTCGTGGTTCGAGCTGGCTTGGCTTGCCGAACAGGAACGCA
TCCAACTGGCGATTCCGGTGCTGTCCTGGCTTCAGCAGCTGGCCGAGCACGTTCGCACCGTCGGTATCACGCCCTCGGTC
GCCGCCACGGCGGTGGCGCTGCCCTCGTCGTTCCCCGGCGATCCGGCCGACCGGTTGATCTACGCCACCGCGATCGAACA
CGGCTGGCGGCTGGTGACCAAGGACCGGCGGCTACGCAGTCATCGGCACCCACGACCGGTCACCGTCTGGTAG

Antitoxin


Download         Length: 72 a.a.        Molecular weight: 7511.55 Da        Isoelectric Point: 4.9732

>AT28776 WP_003414495.1 NC_020089:c3134687-3134472 [Mycobacterium tuberculosis 7199-99]
MTATEVKAKILSLLDEVAQGEEIEITKHGRTVARLVAATGPHALKGRFSGVAMAAVDDDELFTTGVSWNVS

Download         Length: 216 bp

>AT28776 NC_020089:c3134687-3134472 [Mycobacterium tuberculosis 7199-99]
ATGACCGCTACGGAGGTGAAGGCGAAGATCCTCTCCTTGCTTGATGAAGTGGCCCAGGGCGAGGAGATCGAGATCACCAA
ACACGGCCGCACCGTGGCCCGGCTGGTGGCAGCGACGGGGCCGCACGCGCTGAAGGGTCGATTCTCGGGTGTGGCGATGG
CGGCCGTGGATGACGACGAACTCTTCACCACCGGGGTTTCGTGGAACGTTTCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BWM8

Antitoxin

Source ID Structure
AlphaFold DB A0A829CBY8

References