287741

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 254050..254651 Replicon chromosome
Accession NZ_LR134337
Organism Enterococcus faecium strain NCTC7174

Toxin (Protein)


Gene name mazF Uniprot ID A0A0V7YM55
Locus tag EL154_RS01330 Protein ID WP_002330364.1
Coordinates 254307..254651 (+) Length 115 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A6B3Q8V0
Locus tag EL154_RS01325 Protein ID WP_002318881.1
Coordinates 254050..254313 (+) Length 88 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EL154_RS01295 249400..249702 + 303 WP_002305086.1 PTS sugar transporter subunit IIB -
EL154_RS01300 249721..251085 + 1365 WP_002319286.1 PTS sugar transporter subunit IIC -
EL154_RS01305 251141..251455 + 315 WP_002305088.1 PTS lactose/cellobiose transporter subunit IIA -
EL154_RS01310 251789..252835 + 1047 Protein_248 LacI family DNA-binding transcriptional regulator -
EL154_RS01320 253324..253863 - 540 Protein_249 IS30-like element IS6770 family transposase -
EL154_RS01325 254050..254313 + 264 WP_002318881.1 PbsX family transcriptional regulator Antitoxin
EL154_RS01330 254307..254651 + 345 WP_002330364.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
EL154_RS01335 254786..255466 - 681 WP_172594866.1 IS6-like element IS1216 family transposase -
EL154_RS01340 255633..255842 + 210 WP_082194338.1 hypothetical protein -
EL154_RS01345 256049..256249 + 201 Protein_254 hypothetical protein -
EL154_RS01350 256735..256914 + 180 WP_002340501.1 hypothetical protein -
EL154_RS01355 256960..257295 - 336 Protein_256 recombinase family protein -
EL154_RS01360 257315..258356 - 1042 Protein_257 IS630 family transposase -
EL154_RS01365 258434..259422 + 989 Protein_258 IS3 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 254050..270335 16285
- inside IScluster/Tn - - 253315..257803 4488


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(6-111)

Antitoxin

Antitoxin PemI-like

(4-87)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 13024.11 Da        Isoelectric Point: 8.8971

>T287741 WP_002330364.1 NZ_LR134337:254307-254651 [Enterococcus faecium]
MVDVPRQGDILLLNTAPRSGHEQTGRRPYIVLSHDIIADTSNVVIVAPITTTSRNYPLYIEITENHKMKTRGKVMLDQIT
TIDYEARNCKFLEKASEKLIEELLTKIRAVFQKV

Download         Length: 345 bp

Antitoxin


Download         Length: 88 a.a.        Molecular weight: 10071.69 Da        Isoelectric Point: 5.2300

>AT287741 WP_002318881.1 NZ_LR134337:254050-254313 [Enterococcus faecium]
MEMIPIKKWGNSNGLRLPKNLMEFLELKTDDRVSIKSEDVNGKRRLIIEPAISKEEVTIEELFKDYTEGKVNIKIQDLGS
PVGRELW

Download         Length: 264 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0V7YM55

Antitoxin

Source ID Structure
AlphaFold DB A0A6B3Q8V0

References