Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 3109563..3110133 Replicon chromosome
Accession NC_020089
Organism Mycobacterium tuberculosis 7199-99

Toxin (Protein)


Gene name mazF Uniprot ID P71650
Locus tag MT7199_RS14685 Protein ID WP_003414166.1
Coordinates 3109563..3109919 (-) Length 119 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID P0CL61
Locus tag MT7199_RS14690 Protein ID WP_003901465.1
Coordinates 3109903..3110133 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MT7199_RS14665 3105015..3106703 - 1689 WP_003414155.1 alpha/beta hydrolase family protein -
MT7199_RS14670 3106707..3107033 - 327 WP_003414157.1 hypothetical protein -
MT7199_RS14675 3107206..3107793 + 588 WP_003914429.1 DUF3558 family protein -
MT7199_RS14680 3107812..3109461 + 1650 WP_003900576.1 CocE/NonD family hydrolase -
MT7199_RS14685 3109563..3109919 - 357 WP_003414166.1 type II toxin-antitoxin system toxin endoribonuclease MazF9 Toxin
MT7199_RS14690 3109903..3110133 - 231 WP_003901465.1 antitoxin MazE Antitoxin
MT7199_RS14695 3110176..3111219 - 1044 WP_003414172.1 DUF2293 domain-containing protein -
MT7199_RS22240 3111410..3111685 + 276 WP_003911993.1 DUF1778 domain-containing protein -
MT7199_RS22395 3111861..3112115 - 255 WP_003917684.1 hypothetical protein -
MT7199_RS14710 3112263..3112667 + 405 WP_009938577.1 hypothetical protein -
MT7199_RS14715 3112664..3112855 + 192 WP_003414184.1 hypothetical protein -
MT7199_RS14720 3112919..3114208 + 1290 Protein_2892 transposase family protein -
MT7199_RS14725 3114442..3114699 + 258 WP_003899489.1 hypothetical protein -
MT7199_RS14730 3114804..3115115 + 312 WP_003414190.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-113)

Antitoxin

Ribbon-helix-helix protein, CopG

(3-29)


Sequences

Toxin        


Download         Length: 119 a.a.        Molecular weight: 12858.73 Da        Isoelectric Point: 9.1962

>T28774 WP_003414166.1 NC_020089:c3109919-3109563 [Mycobacterium tuberculosis 7199-99]
VMRRGEIWQVDLDPARGSEANNQRPAVVVSNDRANATATRLGRGVITVVPVTSNIAKVYPFQVLLSATTTGLQVDCKAQA
EQIRSIATERLLRPIGRVSAAELAQLDEALKLHLDLWS

Download         Length: 357 bp

>T28774 NC_020089:c3109919-3109563 [Mycobacterium tuberculosis 7199-99]
GTGATGCGCCGCGGTGAGATTTGGCAGGTCGATCTCGACCCCGCTCGAGGTAGCGAAGCGAACAACCAGCGCCCCGCCGT
CGTCGTCAGCAACGACCGGGCCAACGCGACCGCCACGCGTCTTGGGCGCGGCGTCATCACCGTCGTGCCGGTGACGAGCA
ACATCGCCAAGGTCTATCCGTTTCAGGTGTTGTTGTCGGCCACCACTACTGGTCTCCAGGTCGACTGCAAGGCGCAGGCC
GAGCAAATCAGATCGATTGCTACCGAGCGGTTGCTCCGGCCAATCGGCCGAGTTTCAGCCGCCGAACTTGCCCAGCTCGA
TGAGGCTTTGAAACTGCATCTCGACTTATGGTCGTAG

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8231.01 Da        Isoelectric Point: 4.1809

>AT28774 WP_003901465.1 NC_020089:c3110133-3109903 [Mycobacterium tuberculosis 7199-99]
VKLSVSLSDDDVAILDAYVKRAGLPSRSAGLQHAIRVLRYPTLEDDYANAWQEWSAAGDTDAWEQTVGDGVGDAPR

Download         Length: 231 bp

>AT28774 NC_020089:c3110133-3109903 [Mycobacterium tuberculosis 7199-99]
GTGAAGTTGAGCGTGAGCCTGTCCGATGACGACGTCGCGATCCTGGACGCATATGTGAAACGAGCGGGATTACCATCCCG
CTCCGCCGGCCTGCAGCATGCGATCCGCGTGCTTCGCTACCCCACGCTCGAAGATGACTATGCCAACGCATGGCAAGAAT
GGTCGGCAGCCGGCGACACGGACGCGTGGGAGCAGACCGTCGGCGACGGAGTCGGTGATGCGCCGCGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6L2A
PDB 5HJZ
PDB 6KYT
PDB 6L29
PDB 7DU5
PDB 6KYS

Antitoxin

Source ID Structure
PDB 7DU5
PDB 6KYT
AlphaFold DB A0A7U4FBW3

References