Detailed information of TA system TA028773

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 3069979..3070666
• Replicon: chromosome
• Accession: NC_020089
• Organism: Mycobacterium tuberculosis 7199-99

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TF65
• Locus tag: MT7199_RS14475
• Protein ID: WP_003414064.1
• Coordinates: 3070271..3070666 (-)
• Length: 132 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TF64
• Locus tag: MT7199_RS14470
• Protein ID: WP_003414061.1
• Coordinates: 3069979..3070245 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MT7199_RS14445	3065618..3066520	-	903	WP_003900564.1	4-hydroxy-tetrahydrodipicolinate synthase	-
MT7199_RS14450	3066589..3067341	-	753	WP_003899465.1	FAD-dependent thymidylate synthase	-
MT7199_RS14455	3067585..3067860	-	276	WP_003414055.1	type I restriction endonuclease subunit S	-
MT7199_RS14460	3067857..3069479	-	1623	WP_003414057.1	type I restriction-modification system subunit M	-
MT7199_RS14465	3069566..3069982	-	417	WP_003414059.1	type II toxin-antitoxin system toxin ribonuclease C21	-
MT7199_RS14470	3069979..3070245	-	267	WP_003414061.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
MT7199_RS14475	3070271..3070666	-	396	WP_003414064.1	type II toxin-antitoxin system VapC family toxin	Toxin
MT7199_RS14480	3070663..3070932	-	270	WP_003414066.1	type II toxin-antitoxin system VapB family antitoxin	-
MT7199_RS14485	3070942..3072036	-	1095	WP_003900565.1	restriction endonuclease subunit S	-
MT7199_RS14490	3072033..3072452	-	420	WP_003414070.1	winged helix-turn-helix domain-containing protein	-
MT7199_RS14495	3072526..3073005	-	480	WP_003414073.1	dihydrofolate reductase	-
MT7199_RS14500	3073076..3073876	-	801	WP_003911953.1	thymidylate synthase	-
MT7199_RS14505	3074032..3074769	+	738	WP_003414079.1	dienelactone hydrolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-125)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (16-56)

Sequences

Toxin

Protein

• Length: 132 a.a.
• Molecular weight: 14340.04 Da
• Isoelectric Point: 4.7145

>T28773 WP_003414064.1 NC_020089:c3070666-3070271 [Mycobacterium tuberculosis 7199-99]
VIVDTSAIVAIVSGESGAQVLKEALERSPNSRMSAPNYVELCAIMQRRDRPEISRLVDRLLDDYGIQVEAVDADQARVAA
QAYRDYGRGSGHPARLNLGDTYSYALAQVTGEPLLFRGDDFTHTDIRPACT

Nucleotide

• Length: 396 bp

>T28773 NC_020089:c3070666-3070271 [Mycobacterium tuberculosis 7199-99]
GTGATCGTCGATACGTCGGCCATCGTCGCCATCGTGAGCGGGGAATCGGGCGCGCAGGTGCTCAAGGAGGCGCTGGAGCG
GTCACCGAACTCCCGAATGTCCGCGCCCAACTACGTCGAACTGTGCGCGATCATGCAGCGGCGGGACCGGCCGGAGATCT
CTCGATTGGTGGACCGTTTGCTGGACGACTACGGAATCCAGGTCGAAGCCGTCGACGCCGACCAAGCCCGCGTGGCCGCG
CAGGCGTATCGCGACTACGGCCGCGGCAGCGGCCATCCGGCCCGTCTCAACCTCGGCGACACCTACAGCTACGCCCTGGC
CCAGGTCACCGGGGAGCCGCTGCTGTTTCGTGGCGATGACTTCACGCATACCGATATCCGGCCCGCGTGCACCTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9493.77 Da
• Isoelectric Point: 7.2783

>AT28773 WP_003414061.1 NC_020089:c3070245-3069979 [Mycobacterium tuberculosis 7199-99]
MHRGYALVVCSPGVTRTMIDIDDDLLARAAKELGTTTKKDTVHAALRAALRASAARSLMNRMAENATGTQDEALVNAMWR
DGHPENTA

Nucleotide

• Length: 267 bp

>AT28773 NC_020089:c3070245-3069979 [Mycobacterium tuberculosis 7199-99]
ATGCATCGCGGATATGCATTAGTAGTATGCTCTCCGGGTGTGACGCGCACCATGATCGACATCGATGACGACCTGCTCGC
TCGGGCCGCCAAGGAACTGGGTACCACGACAAAGAAAGACACCGTGCATGCCGCGCTGCGGGCCGCGTTGCGAGCCAGTG
CCGCGCGCTCGCTCATGAACCGGATGGCCGAGAACGCCACCGGAACCCAGGACGAAGCTCTGGTCAACGCAATGTGGCGA
GACGGCCACCCAGAGAACACGGCATGA

Structures

Toxin

• AlphaFold DB: G0TF65

Antitoxin

• AlphaFold DB: G0TF64