28769

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 2870342..2870979 Replicon chromosome
Accession NC_020089
Organism Mycobacterium tuberculosis 7199-99

Toxin (Protein)


Gene name vapC Uniprot ID P0DMV7
Locus tag MT7199_RS13400 Protein ID WP_003413180.1
Coordinates 2870342..2870737 (-) Length 132 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ44
Locus tag MT7199_RS13405 Protein ID WP_003413183.1
Coordinates 2870734..2870979 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MT7199_RS13360 2865744..2866955 + 1212 WP_003900845.1 alpha/beta hydrolase family protein -
MT7199_RS13365 2867082..2867741 + 660 WP_003900846.1 LppA family lipoprotein -
MT7199_RS13370 2867738..2868401 + 664 Protein_2621 hypothetical protein -
MT7199_RS22770 2868398..2868676 + 279 WP_003901422.1 type II toxin-antitoxin system VapB family antitoxin -
MT7199_RS13380 2868769..2869182 + 414 WP_003413164.1 PIN domain nuclease -
MT7199_RS13385 2869221..2869478 + 258 WP_003413167.1 ribbon-helix-helix domain-containing protein -
MT7199_RS13390 2869475..2869852 + 378 WP_003413174.1 type II toxin-antitoxin system VapC family toxin -
MT7199_RS13395 2869868..2870242 - 375 WP_003413177.1 hypothetical protein -
MT7199_RS13400 2870342..2870737 - 396 WP_003413180.1 type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20 Toxin
MT7199_RS13405 2870734..2870979 - 246 WP_003413183.1 type II toxin-antitoxin system antitoxin VapB20 Antitoxin
MT7199_RS13410 2871374..2871793 - 420 WP_003413190.1 A24 family peptidase -
MT7199_RS13415 2871805..2872614 - 810 WP_003413193.1 shikimate dehydrogenase -
MT7199_RS13420 2872611..2873864 - 1254 WP_003413196.1 endolytic transglycosylase MltG -
MT7199_RS13425 2873857..2874369 - 513 WP_003413197.1 Holliday junction resolvase RuvX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Endonuclease VapC20-like

(1-129)

Antitoxin

Ribbon-helix-helix protein, CopG

(11-49)


Sequences

Toxin        


Download         Length: 132 a.a.        Molecular weight: 14619.53 Da        Isoelectric Point: 6.9794

>T28769 WP_003413180.1 NC_020089:c2870737-2870342 [Mycobacterium tuberculosis 7199-99]
MIFVDTSFWAALGNAGDARHGTAKRLWASKPPVVMTSNHVLGETWTLLNRRCGHRAAVAAAAIRLSTVVRVEHVTADLEE
QAWEWLVRHDEREYSFVDATSFAVMRKKGIQNAYAFDGDFSAAGFVEVRPE

Download         Length: 396 bp

>T28769 NC_020089:c2870737-2870342 [Mycobacterium tuberculosis 7199-99]
ATGATCTTCGTCGACACGTCCTTCTGGGCTGCACTTGGCAACGCCGGCGACGCTCGGCACGGCACCGCGAAGAGGTTGTG
GGCCAGCAAACCGCCCGTGGTGATGACCTCCAACCATGTCCTGGGCGAGACCTGGACGCTGCTCAACCGGCGCTGCGGTC
ACCGCGCGGCGGTTGCCGCCGCTGCAATCCGCTTGAGCACCGTCGTTCGCGTCGAGCACGTAACAGCCGACCTGGAAGAG
CAGGCATGGGAATGGCTTGTACGCCACGACGAGCGAGAGTACTCGTTCGTCGATGCCACGAGCTTCGCGGTGATGCGAAA
GAAAGGCATCCAAAATGCGTATGCCTTCGATGGTGACTTCAGCGCGGCCGGATTTGTCGAGGTAAGACCCGAGTAG

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 9098.33 Da        Isoelectric Point: 5.6007

>AT28769 WP_003413183.1 NC_020089:c2870979-2870734 [Mycobacterium tuberculosis 7199-99]
MLVAYICHVKRLQIYIDEDVDRALAVEARRRRTSKAALIREYVAEHLRQPGPDPVDAFVGSFVGEADLSASVDDVVYGKH
E

Download         Length: 246 bp

>AT28769 NC_020089:c2870979-2870734 [Mycobacterium tuberculosis 7199-99]
ATGCTAGTGGCGTATATTTGCCATGTGAAAAGGCTGCAGATCTACATCGACGAAGACGTTGACCGAGCGCTTGCTGTTGA
AGCGCGACGGCGGCGGACGTCGAAGGCGGCGCTGATCCGGGAGTACGTCGCTGAGCACCTCCGGCAGCCGGGCCCAGATC
CGGTCGATGCCTTCGTCGGATCGTTCGTGGGGGAAGCCGACTTGTCCGCGTCCGTCGACGACGTGGTTTACGGAAAGCAC
GAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5WZ4
PDB 5WZF

Antitoxin

Source ID Structure
AlphaFold DB A0A7U4BW31

References