Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) itaRT/DUF1778(antitoxin)
Location 3395774..3396611 Replicon chromosome
Accession NZ_LR134315
Organism Escherichia coli strain NCTC9064

Toxin (Protein)


Gene name itaT Uniprot ID Q3Z4X7
Locus tag EL184_RS16655 Protein ID WP_000227784.1
Coordinates 3396069..3396611 (+) Length 181 a.a.

Antitoxin (Protein)


Gene name itaR Uniprot ID I2UQS9
Locus tag EL184_RS16650 Protein ID WP_001297137.1
Coordinates 3395774..3396085 (+) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EL184_RS16625 3390794..3391741 + 948 WP_001239441.1 cytochrome o ubiquinol oxidase subunit II -
EL184_RS16630 3391763..3393754 + 1992 WP_000467180.1 cytochrome o ubiquinol oxidase subunit I -
EL184_RS16635 3393744..3394358 + 615 WP_000179819.1 cytochrome o ubiquinol oxidase subunit III -
EL184_RS16640 3394358..3394687 + 330 WP_000019869.1 cytochrome o ubiquinol oxidase subunit IV -
EL184_RS16645 3394699..3395589 + 891 WP_000971336.1 heme o synthase -
EL184_RS16650 3395774..3396085 + 312 WP_001297137.1 DUF1778 domain-containing protein Antitoxin
EL184_RS16655 3396069..3396611 + 543 WP_000227784.1 GNAT family N-acetyltransferase Toxin
EL184_RS16660 3396667..3397602 - 936 WP_096835275.1 sel1 repeat family protein -
EL184_RS16665 3398010..3399374 + 1365 WP_096835274.1 MFS transporter -
EL184_RS16670 3399502..3399993 - 492 WP_001138904.1 nucleotide binding protein YajQ -
EL184_RS16675 3400161..3401072 + 912 WP_000705865.1 2-dehydropantoate 2-reductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(68-156)

Antitoxin

Vibrio phage ICP1, Orf50

(14-97)


Sequences

Toxin        


Download         Length: 181 a.a.        Molecular weight: 19805.02 Da        Isoelectric Point: 8.3395

>T287651 WP_000227784.1 NZ_LR134315:3396069-3396611 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Download         Length: 543 bp

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11358.04 Da        Isoelectric Point: 10.4825

>AT287651 WP_001297137.1 NZ_LR134315:3395774-3396085 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Download         Length: 312 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7BYY

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y6B3

References