Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 2238744..2239330 Replicon chromosome
Accession NC_020089
Organism Mycobacterium tuberculosis 7199-99

Toxin (Protein)


Gene name mazF Uniprot ID G0TLY3
Locus tag MT7199_RS10410 Protein ID WP_003410010.1
Coordinates 2238744..2239088 (-) Length 115 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID P0CL58
Locus tag MT7199_RS10415 Protein ID WP_003410014.1
Coordinates 2239082..2239330 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MT7199_RS10375 2234450..2235049 + 600 WP_003409989.1 L-lysine exporter -
MT7199_RS10380 2235465..2235893 + 429 WP_003409992.1 cellulose-binding protein -
MT7199_RS10385 2236119..2236658 + 540 WP_003900446.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(37) -
MT7199_RS10390 2237178..2237738 - 561 WP_003410001.1 RES family NAD+ phosphorylase -
MT7199_RS10395 2237735..2238076 - 342 WP_003410003.1 DUF2384 domain-containing protein -
MT7199_RS10400 2238162..2238419 + 258 WP_003410006.1 hypothetical protein -
MT7199_RS10405 2238320..2238655 - 336 WP_003410009.1 dehydrogenase -
MT7199_RS10410 2238744..2239088 - 345 WP_003410010.1 type II toxin-antitoxin system toxin endoribonuclease MazF6 Toxin
MT7199_RS10415 2239082..2239330 - 249 WP_003410014.1 ribbon-helix-helix protein, CopG family Antitoxin
MT7199_RS10420 2239430..2241745 - 2316 WP_003899120.1 cation transporter ATPase CptG -
MT7199_RS10425 2241742..2242014 - 273 WP_003410017.1 DUF1490 family protein -
MT7199_RS10430 2242067..2242423 - 357 WP_003410018.1 Cd(II)/Pb(II)-sensing metalloregulatory transcriptional regulator CmtR -
MT7199_RS10435 2242580..2243347 + 768 WP_003410019.1 hemerythrin domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(5-112)

Antitoxin

Ribbon-helix-helix protein, CopG

(5-38)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 12230.12 Da        Isoelectric Point: 9.8887

>T28759 WP_003410010.1 NC_020089:c2239088-2238744 [Mycobacterium tuberculosis 7199-99]
VVISRAEIYWADLGPPSGSQPAKRRPVLVIQSDPYNASRLATVIAAVITSNTALAAMPGNVFLPATTTRLPRDSVVNVTA
IVTLNKTDLTDRVGEVPASLMHEVDRGLRRVLDL

Download         Length: 345 bp

>T28759 NC_020089:c2239088-2238744 [Mycobacterium tuberculosis 7199-99]
GTGGTGATTAGTCGTGCCGAGATCTACTGGGCTGACCTCGGGCCGCCATCAGGCAGTCAGCCGGCGAAGCGCCGCCCGGT
GCTCGTAATCCAGTCAGATCCGTACAACGCAAGTCGCCTTGCCACTGTGATCGCAGCGGTGATCACGTCCAATACGGCGC
TGGCGGCAATGCCCGGCAACGTGTTCTTGCCCGCGACCACAACGCGACTGCCACGTGACTCGGTCGTCAACGTCACGGCG
ATTGTCACGCTCAACAAGACTGACCTCACCGACCGAGTTGGGGAGGTGCCAGCGAGCTTGATGCACGAGGTTGACCGAGG
ACTTCGTCGCGTACTGGACCTTTGA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9290.34 Da        Isoelectric Point: 4.3757

>AT28759 WP_003410014.1 NC_020089:c2239330-2239082 [Mycobacterium tuberculosis 7199-99]
MKTAISLPDETFDRVSRRASELGMSRSEFFTKAAQRYLHELDAQLLTGQIDRALESIHGTDEAEALAVANAYRVLETMDD
EW

Download         Length: 249 bp

>AT28759 NC_020089:c2239330-2239082 [Mycobacterium tuberculosis 7199-99]
ATGAAGACAGCTATTTCTCTGCCGGATGAGACGTTCGATCGGGTATCGCGGCGTGCGAGTGAGCTCGGCATGAGTCGGTC
CGAGTTCTTCACGAAGGCTGCGCAGCGCTACCTGCACGAGCTGGACGCCCAATTGCTCACGGGCCAGATCGACAGGGCTC
TAGAGAGCATCCATGGCACCGACGAAGCGGAGGCCCTCGCCGTGGCCAACGCATACCGCGTGCTAGAAACCATGGACGAT
GAGTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5HK3
PDB 5HK0
PDB 5HKC

Antitoxin

Source ID Structure
PDB 7WJ0
PDB 7WNR
AlphaFold DB A0A7U4FAW9

References