Detailed information of TA system TA028754

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2199082..2199785
• Replicon: chromosome
• Accession: NC_020089
• Organism: Mycobacterium tuberculosis 7199-99

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G0TLK7
• Locus tag: MT7199_RS10130
• Protein ID: WP_003409778.1
• Coordinates: 2199082..2199411 (-)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G0TLK8
• Locus tag: MT7199_RS21290
• Protein ID: WP_003409780.1
• Coordinates: 2199408..2199785 (-)
• Length: 126 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MT7199_RS10110	2195465..2196535	+	1071	WP_003899091.1	epoxide hydrolase EphB	-
MT7199_RS10115	2196532..2197047	+	516	WP_003409718.1	flavin reductase family protein	-
MT7199_RS10120	2197044..2198105	+	1062	WP_003899092.1	3,4-dihydroxy-2-butanone-4-phosphate synthase	-
MT7199_RS10125	2198102..2198872	+	771	WP_003409775.1	SDR family oxidoreductase	-
MT7199_RS10130	2199082..2199411	-	330	WP_003409778.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
MT7199_RS21290	2199408..2199785	-	378	WP_003409780.1	type II toxin-antitoxin system antitoxin MazE5	Antitoxin
MT7199_RS10140	2199782..2200372	-	591	WP_003409784.1	SEC-C domain-containing protein	-
MT7199_RS10145	2200427..2201791	+	1365	WP_003903691.1	HNH endonuclease	-
MT7199_RS10150	2201946..2202398	-	453	WP_003899095.1	lipoprotein	-
MT7199_RS10155	2202462..2202863	+	402	WP_003409869.1	hypothetical protein	-
MT7199_RS10160	2202856..2203038	-	183	WP_003409870.1	hypothetical protein	-
MT7199_RS10165	2203152..2203502	-	351	WP_003899096.1	hypothetical protein	-
MT7199_RS10170	2203513..2204415	-	903	WP_003899097.1	hypothetical protein	-
MT7199_RS10175	2204436..2204627	-	192	WP_003409876.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-103)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 11787.82 Da
• Isoelectric Point: 8.5361

>T28754 WP_003409778.1 NC_020089:c2199411-2199082 [Mycobacterium tuberculosis 7199-99]
VTALPARGEVWWCEMAEIGRRPVVVLSRDAAIPRLRRALVAPCTTTIRGLASEVVLEPGSDPIPRRSAVNLDSVESVSVA
VLVNRLGRLADIRMRAICTALEVAVDCSR

Nucleotide

• Length: 330 bp

>T28754 NC_020089:c2199411-2199082 [Mycobacterium tuberculosis 7199-99]
GTGACCGCACTTCCGGCGCGCGGAGAGGTGTGGTGGTGTGAGATGGCTGAGATCGGTCGGCGACCAGTCGTCGTGCTGTC
GCGCGATGCCGCGATCCCTCGGCTGCGACGCGCACTTGTCGCGCCCTGCACCACGACCATCCGAGGGCTAGCCAGTGAGG
TTGTTCTTGAACCCGGTTCCGACCCGATCCCGCGCCGTTCCGCGGTGAATTTGGACTCAGTCGAAAGTGTCTCGGTCGCG
GTATTGGTGAATCGGCTTGGCCGCCTCGCCGACATCCGGATGCGCGCCATCTGCACGGCCCTCGAGGTCGCCGTCGATTG
CTCTCGATGA

Antitoxin

Protein

• Length: 126 a.a.
• Molecular weight: 13560.07 Da
• Isoelectric Point: 5.1519

>AT28754 WP_003409780.1 NC_020089:c2199785-2199408 [Mycobacterium tuberculosis 7199-99]
VKTARLQVTLRCAVDLINSSSDQCFARIEHVASDQADPRPGVWHSSGMNRIRLSTTVDAALLTSARDMRAGITDAALIDE
ALAALLARHRSAEVDASYAAYDKHPVDEPDEWGDLASWRRAAGDS

Nucleotide

• Length: 378 bp

>AT28754 NC_020089:c2199785-2199408 [Mycobacterium tuberculosis 7199-99]
GTGAAGACGGCCCGGTTGCAGGTGACGTTGCGCTGCGCGGTAGACCTCATCAATTCGTCCAGCGACCAATGTTTCGCCCG
TATCGAGCACGTTGCGTCCGATCAGGCGGATCCACGCCCCGGAGTGTGGCATAGTAGTGGCATGAATCGCATACGGCTGA
GCACTACTGTCGACGCCGCACTCTTGACAAGTGCGCGCGACATGCGGGCAGGAATCACTGACGCAGCCCTCATCGATGAG
GCCCTTGCGGCATTACTGGCTCGTCATCGATCGGCCGAGGTGGATGCCAGCTATGCGGCCTACGACAAGCACCCGGTCGA
TGAGCCCGACGAGTGGGGCGATCTAGCGTCGTGGCGGCGGGCGGCTGGAGACTCGTGA

Structures

Toxin

• AlphaFold DB: G0TLK7

Antitoxin

• AlphaFold DB: G0TLK8