Detailed information of TA system TA287530

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mosAT/AbiEii-AbiEi
• Location: 3345724..3347446
• Replicon: chromosome
• Accession: NZ_LR134303
• Organism: Shewanella putrefaciens strain NCTC12093

Toxin (Protein)

• Gene name: mosT
• Uniprot ID: D0FZT9
• Locus tag: EL161_RS14685
• Protein ID: WP_000356587.1
• Coordinates: 3346508..3347446 (+)
• Length: 313 a.a.

Antitoxin (Protein)

• Gene name: mosA
• Uniprot ID: -
• Locus tag: EL161_RS14680
• Protein ID: WP_048608544.1
• Coordinates: 3345724..3346515 (+)
• Length: 264 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EL161_RS14650	3341397..3341678	+	282	WP_000433889.1	type IV conjugative transfer system protein TraL	-
EL161_RS14655	3341675..3342301	+	627	WP_000667170.1	hypothetical protein	-
EL161_RS14660	3342285..3343181	+	897	WP_048608549.1	type-F conjugative transfer system secretin TraK	-
EL161_RS14665	3343184..3344473	+	1290	WP_085285719.1	TraB/VirB10 family protein	-
EL161_RS14670	3344548..3345120	+	573	WP_139175592.1	type IV conjugative transfer system lipoprotein TraV	-
EL161_RS14675	3345117..3345503	+	387	WP_048608546.1	hypothetical protein	-
EL161_RS14680	3345724..3346515	+	792	WP_048608544.1	type IV toxin-antitoxin system AbiEi family antitoxin	Antitoxin
EL161_RS14685	3346508..3347446	+	939	WP_000356587.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
EL161_RS14690	3347578..3348270	+	693	WP_001228924.1	DsbC family protein	-
EL161_RS14695	3348270..3350669	+	2400	WP_048608542.1	type IV secretion system protein TraC	-
EL161_RS14700	3350662..3351009	+	348	WP_048608540.1	hypothetical protein	-
EL161_RS14705	3350993..3351505	+	513	WP_172689549.1	S26 family signal peptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	floR / tet(G) / sul2 / aph(3'')-Ib / aph(6)-Id	-	3284772..3347446	62674

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (14-278)

Antitoxin

• Transcriptional regulator AbiEi antitoxin, N-terminal domain (1-97)

Sequences

Toxin

Protein

• Length: 313 a.a.
• Molecular weight: 35548.82 Da
• Isoelectric Point: 4.9081

>T287530 WP_000356587.1 NZ_LR134303:3346508-3347446 [Shewanella putrefaciens]
MDKDSPYYKQVSLLIRMLPVVATETVFALKGGTAINLFVRDFPRLSVDIDLAYLPLEPRDEALINVRAALQRITDRINTQ
PDIRAAFQDNKADELRIVVSSPVATIKIEVSPVARGTLHSAEIMPVQESVEDEFGYAEIQVVSLPDLYGGKLCAAMDRQH
PRDLFDVRMLLGSEGISREILVGFLTYTLSHPRPINEVMSPNWQPLNEKFQAEFDGMTFEKVECEDLASVRPMMLIELQK
HFTERDHAFLMSFKRGQPDWALFDYPNAADLPAIRWKLQNINKLAKNQAKHQEQLDKLKQVLDDWLVNANAE

Nucleotide

• Length: 939 bp

Antitoxin

Protein

• Length: 264 a.a.
• Molecular weight: 30100.44 Da
• Isoelectric Point: 9.3513

>AT287530 WP_048608544.1 NZ_LR134303:3345724-3346515 [Shewanella putrefaciens]
MSSKINWLVAHTSPGALVLQQWLTENGVSYSLAQKYAQNGWLKKLSSGVYYRPDAQGDIKPTWVDAIQALDVQLGVSVHL
AGLSSLTHQGLSHYLQLNKEQVWICVKNKSSLPKWFREFPYQNWFYCGNHKLEVNPEKDLKRITVKEKELTVSCAELAAY
EVVDAIGKLISFEHVAELFQGLVNLSPRKVQDILERSSSVQANRIFLFLGRYYDHQWVNRIDETRIKLGAGKRQVVEKGR
FDERYQITVPEILSVKKGEQHNG

Nucleotide

• Length: 792 bp

Structures

Toxin

• AlphaFold DB: A0A1U9VZ44