Detailed information of TA system TA287520

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): rlegTA/AbiEii-AbiEi
• Location: 245124..246655
• Replicon: chromosome
• Accession: NZ_LR134302
• Organism: Achromobacter spanius strain NCTC13519

Toxin (Protein)

• Gene name: rlegT
• Uniprot ID: -
• Locus tag: EL258_RS01130
• Protein ID: WP_157814471.1
• Coordinates: 245124..246035 (-)
• Length: 304 a.a.

Antitoxin (Protein)

• Gene name: rlegA
• Uniprot ID: -
• Locus tag: EL258_RS01135
• Protein ID: WP_100855374.1
• Coordinates: 246041..246655 (-)
• Length: 205 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EL258_RS01105	240268..241023	-	756	WP_100855380.1	ABC transporter ATP-binding protein	-
EL258_RS01110	241016..241984	-	969	WP_100855379.1	branched-chain amino acid ABC transporter permease	-
EL258_RS01115	241987..242859	-	873	WP_100855378.1	branched-chain amino acid ABC transporter permease	-
EL258_RS01120	242869..244074	-	1206	WP_100855377.1	ABC transporter substrate-binding protein	-
EL258_RS01125	244093..244890	-	798	WP_100855376.1	GntR family transcriptional regulator	-
EL258_RS01130	245124..246035	-	912	WP_157814471.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
EL258_RS01135	246041..246655	-	615	WP_100855374.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	Antitoxin
EL258_RS01140	246747..250802	+	4056	WP_100855373.1	helix-turn-helix domain-containing protein	-
EL258_RS01145	250855..251310	+	456	WP_100855372.1	GNAT family N-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	245124..272001	26877

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (33-267)

Antitoxin

• Transcriptional regulator, AbiEi antitoxin (10-55)

Sequences

Toxin

Protein

• Length: 304 a.a.
• Molecular weight: 34299.45 Da
• Isoelectric Point: 7.3840

>T287520 WP_157814471.1 NZ_LR134302:c246035-245124 [Achromobacter spanius]
MNPAFSIRARLLTHAKRHGDDFQRVLTRYAIERLLFRLSQTKVGEHYVLKGAMLFVTWPRYVFRPTGDLDLLGQGNSEPA
ALVELFTRICQVECVADGIVFDAAMVRVEAVREADKYHGSRISLAADLAGARIPVQIDVGFGDRVYPEPKREIFPSLVPD
VPHASILMYPPETVVAEKFEAMIRFGETNTRIKDYYDVWVTTRIFPFDMANVVEAVGGTLRRRETAIPTEMPMGLTSTFA
AIVDERGLWSGFLRRTSPTLTPPSFPDLQSELRSFFRLVIEGLNMSEGARGVWDTDGRVWRNR

Nucleotide

• Length: 912 bp

Antitoxin

Protein

• Length: 205 a.a.
• Molecular weight: 22556.13 Da
• Isoelectric Point: 10.0411

>AT287520 WP_100855374.1 NZ_LR134302:c246655-246041 [Achromobacter spanius]
MSEYPISNRERVLQLVRDRGIARARDLTAAGIPLAYLKRLTDSGDLIRLGRGLYQTPDRVGEDAAHDLAEAARRVPKGVI
SLISALRWHEVTTQLPNAVWMTIPHKSRVPKSTGLRLEIVRATGETLTAGVEYVDVEGVAVPVYCVAKTVADCFKHRRHV
GEDVAIEALRDALHQRKATPSDLMKFAAINRVAGLMAPYIRAMQ

Nucleotide

• Length: 615 bp