287512

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) PP_4151-4152/excise(antitoxin)
Location 1222335..1223377 Replicon chromosome
Accession NZ_LR134300
Organism Pseudomonas fluorescens strain NCTC10783

Toxin (Protein)


Gene name PP_4152 Uniprot ID -
Locus tag EL286_RS05890 Protein ID WP_003109777.1
Coordinates 1222802..1223377 (+) Length 192 a.a.

Antitoxin (Protein)


Gene name PP_4151 Uniprot ID I3TV68
Locus tag EL286_RS05885 Protein ID WP_003050245.1
Coordinates 1222335..1222805 (+) Length 157 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EL286_RS05850 1217727..1219145 - 1419 WP_020924934.1 TIGR03752 family integrating conjugative element protein -
EL286_RS05855 1219135..1220046 - 912 WP_003105643.1 TIGR03749 family integrating conjugative element protein -
EL286_RS05860 1220043..1220735 - 693 WP_003105641.1 TIGR03746 family integrating conjugative element protein -
EL286_RS05865 1220732..1221130 - 399 WP_003105639.1 TIGR03750 family conjugal transfer protein -
EL286_RS05870 1221142..1221501 - 360 WP_003090173.1 TIGR03745 family integrating conjugative element membrane protein -
EL286_RS05875 1221518..1221751 - 234 WP_003050225.1 TIGR03758 family integrating conjugative element protein -
EL286_RS05880 1221748..1222131 - 384 WP_003120001.1 RAQPRD family integrative conjugative element protein -
EL286_RS05885 1222335..1222805 + 471 WP_003050245.1 helix-turn-helix domain-containing protein Antitoxin
EL286_RS05890 1222802..1223377 + 576 WP_003109777.1 PIN domain-containing protein Toxin
EL286_RS05895 1223395..1224309 + 915 WP_003105629.1 AAA family ATPase -
EL286_RS05905 1224673..1225128 + 456 WP_023111669.1 hypothetical protein -
EL286_RS05910 1225128..1226030 + 903 WP_003105624.1 nucleotidyltransferase domain-containing protein -
EL286_RS05915 1226069..1226794 + 726 WP_003050273.1 CBASS effector endonuclease NucC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - - 1175808..1272762 96954


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(9-114)

Antitoxin

Helix-turn-helix domain, group 17

(85-133)


Sequences

Toxin        


Download         Length: 192 a.a.        Molecular weight: 21644.79 Da        Isoelectric Point: 5.6172

>T287512 WP_003109777.1 NZ_LR134300:1222802-1223377 [Pseudomonas fluorescens]
MRHSPFTAVYDACVLYPAPLRDFLMWLGLSGRFRARWSQAIHEEWKRNLLINRPDLTRVQVDRTSDLMDRAIPDGLVEGY
EALVAGLTLPDPDDRHVLAAAIRCGASVIVTFNERDFPNDLLAPYGIESQHPDEFVDNLLDLDAAAVVSAAQRQRAQLKH
PPIEVDRYLEILLRQGLVQTTKVLATYRTIL

Download         Length: 576 bp

Antitoxin


Download         Length: 157 a.a.        Molecular weight: 17245.62 Da        Isoelectric Point: 6.3803

>AT287512 WP_003050245.1 NZ_LR134300:1222335-1222805 [Pseudomonas fluorescens]
MTTATAQSKMTLPAAGEVKAAVQGQRALAAYLATQFETQHIQIFDDHKQAHQVELPTSALRLLVDILAELADGNAVKVVP
VHAELTTQEAADLLNVSRPHFVKLLEDGVLAFHRTGKHRRVRFADLMQYKEARERASEQAMAELAQQSQELGMGYE

Download         Length: 471 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB I3TV68

References