Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relE-higA/HigB-HigA
Location 327688..328556 Replicon chromosome
Accession NZ_LR134299
Organism Pseudomonas putida strain NCTC13186

Toxin (Protein)


Gene name relE Uniprot ID -
Locus tag EL224_RS01445 Protein ID WP_061405554.1
Coordinates 327688..328041 (+) Length 118 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID Q88R60
Locus tag EL224_RS01450 Protein ID WP_004575922.1
Coordinates 328083..328556 (+) Length 158 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EL224_RS01425 322708..324075 - 1368 WP_010951639.1 HAMP domain-containing protein -
EL224_RS01430 324077..324796 - 720 WP_010951640.1 response regulator -
EL224_RS01435 324937..327234 + 2298 WP_010951641.1 TonB-dependent siderophore receptor -
EL224_RS01440 327300..327506 - 207 WP_014860378.1 DUF3079 domain-containing protein -
EL224_RS01445 327688..328041 + 354 WP_061405554.1 type II toxin-antitoxin system HigB family toxin Toxin
EL224_RS01450 328083..328556 + 474 WP_004575922.1 transcriptional regulator Antitoxin
EL224_RS01455 328561..328748 - 188 Protein_273 integrase -
EL224_RS01460 328976..329191 + 216 WP_010951644.1 type II toxin-antitoxin system RelE/ParE family toxin -
EL224_RS01465 329188..329511 - 324 WP_010951645.1 helix-turn-helix domain-containing protein -
EL224_RS01475 329973..330221 - 249 WP_010951646.1 hypothetical protein -
EL224_RS01480 330742..331017 + 276 WP_049588069.1 DUF3077 domain-containing protein -
EL224_RS01485 331372..332577 - 1206 WP_010951648.1 methyltransferase -
EL224_RS01490 332706..333395 - 690 WP_010951649.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(73-111)

Antitoxin

Antitoxin HigA

(43-154)


Sequences

Toxin        


Download         Length: 118 a.a.        Molecular weight: 13672.88 Da        Isoelectric Point: 10.6182

>T287503 WP_061405554.1 NZ_LR134299:327688-328041 [Pseudomonas putida]
MRVITKAAVTKAIEVHGQWKAPLSLWLTTFDRATLRFESFEQLRQTWATVSGWNVDRIPHSKLRPASRKGPLDIYVFDIK
KNECRLIAWLNARTGTLYIKAILSHAEYDKWCRSDIR

Download         Length: 354 bp

Antitoxin


Download         Length: 158 a.a.        Molecular weight: 17229.67 Da        Isoelectric Point: 4.4982

>AT287503 WP_004575922.1 NZ_LR134299:328083-328556 [Pseudomonas putida]
MKRDKIEANGLDAFIANISPMLDGLNTQMATMAQLLAACHDPIKDDAELEARMALIDELYSHADSEGHAAARFAEMVADR
VYEYESESVLVPFASQAEALAFLLSDRGVKQKDLSAIATQSAVSEILNNKRKMTVAQIKGFAEFFSVPVEFFMHGVV

Download         Length: 474 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB A0A2I0CBJ4

References