Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4009438..4010014 Replicon chromosome
Accession NZ_LR134295
Organism Escherichia coli strain NCTC9080

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag EL145_RS19280 Protein ID WP_001297643.1
Coordinates 4009727..4010014 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A829G8Z0
Locus tag EL145_RS19275 Protein ID WP_000063156.1
Coordinates 4009438..4009740 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EL145_RS19260 4006034..4008184 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
EL145_RS19265 4008234..4008437 + 204 WP_000467860.1 YbdD/YjiX family protein -
EL145_RS19270 4008448..4009404 + 957 WP_001297640.1 GTPase -
EL145_RS19275 4009438..4009740 - 303 WP_000063156.1 BrnA antitoxin family protein Antitoxin
EL145_RS19280 4009727..4010014 - 288 WP_001297643.1 BrnT family toxin Toxin
EL145_RS19285 4010213..4010909 - 697 Protein_3751 IS1 family transposase -
EL145_RS19290 4011224..4012456 + 1233 WP_001825915.1 multidrug efflux MFS transporter MdtM -
EL145_RS19295 4012497..4013777 + 1281 WP_001037380.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 4010213..4010422 209


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T287481 WP_001297643.1 NZ_LR134295:c4010014-4009727 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.99 Da        Isoelectric Point: 9.4646

>AT287481 WP_000063156.1 NZ_LR134295:c4009740-4009438 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A829G8Z0

References