Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 3958188..3958813 Replicon chromosome
Accession NZ_LR134280
Organism Klebsiella pneumoniae strain NCTC9793

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag EL169_RS19845 Protein ID WP_002882817.1
Coordinates 3958188..3958571 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag EL169_RS19850 Protein ID WP_004150355.1
Coordinates 3958571..3958813 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EL169_RS19830 3955554..3956456 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
EL169_RS19835 3956453..3957088 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
EL169_RS19840 3957085..3958014 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
EL169_RS19845 3958188..3958571 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
EL169_RS19850 3958571..3958813 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
EL169_RS19855 3959018..3959935 + 918 WP_023313778.1 alpha/beta hydrolase -
EL169_RS19860 3959950..3960891 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
EL169_RS19865 3960936..3961373 - 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
EL169_RS19870 3961370..3962230 - 861 WP_004146232.1 virulence factor BrkB family protein -
EL169_RS19875 3962224..3962823 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T287450 WP_002882817.1 NZ_LR134280:c3958571-3958188 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT287450 WP_004150355.1 NZ_LR134280:c3958813-3958571 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References