Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 3354516..3355326 Replicon chromosome
Accession NZ_LR134280
Organism Klebsiella pneumoniae strain NCTC9793

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag EL169_RS16900 Protein ID WP_004178461.1
Coordinates 3354516..3355049 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag EL169_RS16905 Protein ID WP_002887278.1
Coordinates 3355060..3355326 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EL169_RS16895 3353347..3354468 + 1122 WP_016946712.1 cupin domain-containing protein -
EL169_RS16900 3354516..3355049 - 534 WP_004178461.1 GNAT family N-acetyltransferase Toxin
EL169_RS16905 3355060..3355326 - 267 WP_002887278.1 DUF1778 domain-containing protein Antitoxin
EL169_RS16910 3355429..3356862 - 1434 WP_040236876.1 Cu(+)/Ag(+) sensor histidine kinase -
EL169_RS16915 3356852..3357535 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
EL169_RS16925 3357707..3359089 + 1383 WP_040236875.1 Cu(I)/Ag(I) efflux RND transporter outer membrane protein -
EL169_RS16930 3359107..3359451 + 345 WP_004186842.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T287447 WP_004178461.1 NZ_LR134280:c3355049-3354516 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT287447 WP_002887278.1 NZ_LR134280:c3355326-3355060 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References