Detailed information of TA system TA028741

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): unclassified/Polyketide_cyc2(toxin)
• Location: 1017997..1018616
• Replicon: chromosome
• Accession: NC_020089
• Organism: Mycobacterium tuberculosis 7199-99

Toxin (Protein)

• Gene name: novel[antitoxin]
• Uniprot ID: -
• Locus tag: MT7199_RS04785
• Protein ID: WP_003404726.1
• Coordinates: 1018182..1018616 (+)
• Length: 145 a.a.

Antitoxin (Protein)

• Gene name: novel[toxin]
• Uniprot ID: P9WJ06
• Locus tag: MT7199_RS04780
• Protein ID: WP_003898641.1
• Coordinates: 1017997..1018176 (+)
• Length: 60 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MT7199_RS04770	1013470..1015050	+	1581	WP_003909313.1	serine hydrolase	-
MT7199_RS04775	1015047..1017440	+	2394	WP_003898639.1	cation-translocating P-type ATPase	-
MT7199_RS22545	1017713..1017871	+	159	WP_003404720.1	hypothetical protein	-
MT7199_RS04780	1017997..1018176	+	180	WP_003898641.1	antitoxin	Antitoxin
MT7199_RS04785	1018182..1018616	+	435	WP_003404726.1	SRPBCC family protein	Toxin
MT7199_RS04790	1018714..1019487	+	774	WP_003404735.1	VOC family protein	-
MT7199_RS04795	1019552..1020001	+	450	WP_003404738.1	hypothetical protein	-
MT7199_RS04800	1020136..1020366	-	231	WP_003898642.1	hypothetical protein	-
MT7199_RS04805	1020533..1022041	-	1509	WP_003404742.1	carotenoid oxygenase family protein	-
MT7199_RS04810	1022043..1023281	-	1239	WP_003900233.1	acetyl-CoA acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polyketide cyclase/dehydrase (1-142)

Antitoxin

• Antitoxin Rv0909/MT0933 (4-50)

Sequences

Toxin

Protein

• Length: 145 a.a.
• Molecular weight: 15754.47 Da
• Isoelectric Point: 9.7977

>T28741 WP_003404726.1 NC_020089:1018182-1018616 [Mycobacterium tuberculosis 7199-99]
MAKLSGSIDVPLPPEEAWMHASDLTRYREWLTIHKVWRSKLPEVLEKGTVVESYVEVKGMPNRIKWTIVRYKPPEGMTLN
GDGVGGVKVKLIAKVAPKEHGSVVSFDVHLGGPALLGPIGMIVAAALRADIRESLQNFVTVFAG

Nucleotide

• Length: 435 bp

>T28741 NC_020089:1018182-1018616 [Mycobacterium tuberculosis 7199-99]
ATGGCGAAACTGTCCGGATCCATCGACGTACCGCTGCCACCGGAGGAAGCCTGGATGCACGCCTCCGATCTGACTCGTTA
CCGAGAGTGGCTGACCATCCACAAGGTATGGCGCAGCAAGTTGCCCGAAGTGCTCGAGAAGGGCACGGTCGTCGAGTCGT
ATGTCGAGGTCAAGGGCATGCCCAACCGGATCAAGTGGACGATCGTGCGGTACAAACCCCCGGAGGGCATGACGCTCAAC
GGCGACGGTGTGGGTGGTGTCAAAGTCAAGCTGATCGCTAAGGTAGCGCCGAAAGAGCACGGCTCCGTCGTCAGCTTCGA
TGTGCACCTCGGCGGCCCGGCCCTGCTCGGGCCGATCGGCATGATCGTCGCCGCTGCATTGCGAGCCGACATCCGCGAAT
CGCTGCAGAACTTCGTCACGGTGTTTGCCGGCTGA

Antitoxin

Protein

• Length: 60 a.a.
• Molecular weight: 6403.07 Da
• Isoelectric Point: 4.5393

>AT28741 WP_003898641.1 NC_020089:1017997-1018176 [Mycobacterium tuberculosis 7199-99]
MGILDKVKNLLSQNADKVETVINKAGEFVDEQTQGNYSDAIHKLHDAASNVVGMSDQQS

Nucleotide

• Length: 180 bp

>AT28741 NC_020089:1017997-1018176 [Mycobacterium tuberculosis 7199-99]
ATGGGAATCCTGGACAAGGTAAAGAACCTGCTGTCGCAGAACGCCGACAAGGTCGAGACGGTGATCAACAAAGCGGGCGA
ATTCGTCGACGAGCAGACGCAAGGCAATTATTCTGACGCCATCCACAAGCTGCATGACGCGGCCAGCAACGTCGTCGGCA
TGAGCGACCAGCAGAGCTAG

Structures

Antitoxin

• AlphaFold DB: A0A045KDK5