Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 2250657..2252245 Replicon chromosome
Accession NZ_LR134248
Organism Escherichia coli strain NCTC10537

Toxin (Protein)


Gene name hipA Uniprot ID S1ELM3
Locus tag EL141_RS10945 Protein ID WP_001125439.1
Coordinates 2250923..2252245 (+) Length 441 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID -
Locus tag EL141_RS10940 Protein ID WP_001301023.1
Coordinates 2250657..2250923 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EL141_RS10915 2245832..2246122 - 291 WP_000558527.1 (4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione isomerase -
EL141_RS10920 2246146..2247021 - 876 WP_000774188.1 3-hydroxy-5-phosphonooxypentane-2,4-dione thiolase -
EL141_RS10925 2247048..2248070 - 1023 WP_000172488.1 autoinducer 2 ABC transporter substrate-binding protein LsrB -
EL141_RS10930 2248082..2248858 - 777 Protein_2117 histidine kinase -
EL141_RS10935 2248854..2250434 + 1581 Protein_2118 autotransporter outer membrane beta-barrel domain-containing protein -
EL141_RS10940 2250657..2250923 + 267 WP_001301023.1 type II toxin-antitoxin system antitoxin HipB Antitoxin
EL141_RS10945 2250923..2252245 + 1323 WP_001125439.1 type II toxin-antitoxin system serine/threonine protein kinase toxin HipA Toxin
EL141_RS10950 2252617..2253099 + 483 WP_000931227.1 fimbrial protein -
EL141_RS10955 2253460..2254170 + 711 Protein_2122 fimbria/pilus periplasmic chaperone -
EL141_RS22480 2254212..2256862 + 2651 Protein_2123 fimbrial biogenesis outer membrane usher protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA-like, C-terminal

(149-401)

Antitoxin

Cro/C1-type helix-turn-helix domain

(14-69)


Sequences

Toxin        


Download         Length: 441 a.a.        Molecular weight: 49275.82 Da        Isoelectric Point: 8.2482

>T287386 WP_001125439.1 NZ_LR134248:2250923-2252245 [Escherichia coli]
MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLSLPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLIPEDETVTHPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGNVRALAVERFDRRW
NAERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIARIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLRFPEVQMHEILSDFARMIP
AALDNVKTSLPTDFPENVVTAVESNVLRLHGRLSREYGSK

Download         Length: 1323 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10016.38 Da        Isoelectric Point: 6.2267

>AT287386 WP_001301023.1 NZ_LR134248:2250657-2250923 [Escherichia coli]
MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPDNTTLTTFFKILQSLELSMTLCDAKNASPES
TEQQNLEW

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5K98
PDB 3TPV
PDB 3DNV
PDB 3TPD
PDB 3HZI
PDB 3FBR
PDB 4YG7
PDB 3TPE
PDB 2WIU
PDB 3DNT
PDB 3TPB
PDB 3TPT
PDB 3DNU

Antitoxin

Source ID Structure

References