287365

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 2529545..2530348 Replicon chromosome
Accession NZ_LR134247
Organism Escherichia coli strain NCTC9040

Toxin (Protein)


Gene name KacT Uniprot ID A0A8B2S6X5
Locus tag EL149_RS12700 Protein ID WP_048213819.1
Coordinates 2529545..2530075 (-) Length 177 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID A0A8B2S700
Locus tag EL149_RS12705 Protein ID WP_048213818.1
Coordinates 2530079..2530348 (-) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EL149_RS12670 2525062..2525343 - 282 WP_000639434.1 hypothetical protein -
EL149_RS12675 2525492..2525737 + 246 WP_160448951.1 hypothetical protein -
EL149_RS12680 2525795..2526583 - 789 WP_001543756.1 site-specific integrase -
EL149_RS25100 2526603..2527274 - 672 WP_000796235.1 hypothetical protein -
EL149_RS12690 2528206..2528451 + 246 WP_048213821.1 hypothetical protein -
EL149_RS12695 2528456..2529460 + 1005 WP_048213820.1 hypothetical protein -
EL149_RS12700 2529545..2530075 - 531 WP_048213819.1 GNAT family N-acetyltransferase Toxin
EL149_RS12705 2530079..2530348 - 270 WP_048213818.1 DUF1778 domain-containing protein Antitoxin
EL149_RS12710 2530563..2531060 - 498 Protein_2457 IS3 family transposase -
EL149_RS12715 2531141..2532649 - 1509 WP_126325821.1 group II intron reverse transcriptase/maturase -
EL149_RS12725 2533322..2534007 - 686 Protein_2459 IS3 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 2524584..2560604 36020
- flank IS/Tn - - 2524584..2524961 377


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(92-156)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 177 a.a.        Molecular weight: 20322.17 Da        Isoelectric Point: 6.7544

>T287365 WP_048213819.1 NZ_LR134247:c2530075-2529545 [Escherichia coli]
MDGLRIEIFSEEVEYELSNFDCGEEYLNTFLTDHLKRQHNSKILRGYLLVTREDKPRVMGYYTLSGSCFEKTLLPSKTQQ
KRVPYKNVPSVTLGRLAIDKSIHHQGYGETLVTHAMKVVYQASQAVGIHGMFVEALNDNAKKFYLRLGFIQLKEENSNSL
FYPTKSVEELFEVKDE

Download         Length: 531 bp

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10285.70 Da        Isoelectric Point: 5.7252

>AT287365 WP_048213818.1 NZ_LR134247:c2530348-2530079 [Escherichia coli]
MSALKKQRIDLRLNEDDKHMIEEAAAMTNQSISQFMVSTASERAAEVIDQHRRLLLNEESWNLVMDAITNPPVPNDRLKR
AANRLRELE

Download         Length: 270 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A8B2S6X5

Antitoxin

Source ID Structure
AlphaFold DB A0A8B2S700

References