Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4052436..4053012 Replicon chromosome
Accession NZ_LR134240
Organism Escherichia coli strain NCTC9107

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag EL129_RS19805 Protein ID WP_001297643.1
Coordinates 4052725..4053012 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A080I7H7
Locus tag EL129_RS19800 Protein ID WP_000063160.1
Coordinates 4052436..4052738 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EL129_RS19785 4049032..4051182 + 2151 WP_001340937.1 pyruvate/proton symporter BtsT -
EL129_RS19790 4051232..4051435 + 204 WP_000467859.1 YbdD/YjiX family protein -
EL129_RS19795 4051446..4052402 + 957 WP_001297640.1 GTPase -
EL129_RS19800 4052436..4052738 - 303 WP_000063160.1 BrnA antitoxin family protein Antitoxin
EL129_RS19805 4052725..4053012 - 288 WP_001297643.1 BrnT family toxin Toxin
EL129_RS19810 4053211..4053907 - 697 Protein_3835 IS1 family transposase -
EL129_RS19815 4054221..4055453 + 1233 WP_001137031.1 multidrug efflux MFS transporter MdtM -
EL129_RS19820 4055494..4056774 + 1281 WP_001037390.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 4053211..4053420 209


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T287340 WP_001297643.1 NZ_LR134240:c4053012-4052725 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11552.01 Da        Isoelectric Point: 9.4646

>AT287340 WP_000063160.1 NZ_LR134240:c4052738-4052436 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALTNKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A080I7H7

References