Detailed information of TA system TA028732

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC(toxin)
• Location: 707456..708099
• Replicon: chromosome
• Accession: NC_020089
• Organism: Mycobacterium tuberculosis 7199-99

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P67239
• Locus tag: MT7199_RS03180
• Protein ID: WP_003403187.1
• Coordinates: 707698..708099 (+)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TQ91
• Locus tag: MT7199_RS03175
• Protein ID: WP_003403184.1
• Coordinates: 707456..707701 (+)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MT7199_RS03140	702736..703266	-	531	WP_003917320.1	two component system sensor kinase HK2	-
MT7199_RS03145	703250..703945	-	696	WP_003918099.1	two-component system response regulator TcrA	-
MT7199_RS03150	704068..704379	+	312	WP_003403164.1	hypothetical protein	-
MT7199_RS03155	704451..705401	+	951	WP_003900184.1	DUF1259 domain-containing protein	-
MT7199_RS03160	705642..706226	+	585	WP_003403171.1	IS607-like element IS1536 family transposase	-
MT7199_RS03165	706228..706938	+	711	Protein_629	transposase	-
MT7199_RS03170	706941..707411	+	471	WP_003898523.1	hypothetical protein	-
MT7199_RS03175	707456..707701	+	246	WP_003403184.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
MT7199_RS03180	707698..708099	+	402	WP_003403187.1	type II toxin-antitoxin system VapC family toxin	Toxin
MT7199_RS22130	708487..708654	-	168	WP_077385191.1	DUF3800 domain-containing protein	-
MT7199_RS22135	708687..708884	-	198	WP_003403191.1	hypothetical protein	-
MT7199_RS03195	708964..710121	-	1158	WP_003898524.1	hypothetical protein	-
MT7199_RS03200	710173..710394	-	222	WP_003898525.1	DUF4926 domain-containing protein	-
MT7199_RS03205	710536..711141	+	606	WP_003898526.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-124)

Antitoxin

• Antitoxin VapB-like (1-80)

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 14501.21 Da
• Isoelectric Point: 4.4463

>T28732 WP_003403187.1 NC_020089:707698-708099 [Mycobacterium tuberculosis 7199-99]
VIVDTSAIIAILRDEDDAAAYADALANADVRRLSAASYLECGIVLDSQRDPVISRALDELIEEAEFVVEPVTERQARLAR
AAYADFGRGSGHPAGLNFGDCLSYALAIDRREPLLWKGNDFGHTGVQRALDRR

Nucleotide

• Length: 402 bp

>T28732 NC_020089:707698-708099 [Mycobacterium tuberculosis 7199-99]
GTGATCGTCGACACGTCGGCGATCATCGCGATTCTGCGCGACGAGGACGACGCCGCGGCCTACGCCGACGCGCTCGCCAA
CGCCGATGTCCGCAGACTGTCTGCGGCCAGCTACCTGGAATGCGGGATAGTCCTTGACTCCCAGCGTGATCCGGTCATCA
GCAGAGCACTGGATGAACTTATCGAAGAAGCCGAGTTCGTCGTCGAGCCGGTAACCGAGCGCCAGGCCCGCCTGGCCCGA
GCGGCCTACGCGGATTTCGGCAGAGGCAGCGGCCACCCCGCGGGCTTGAATTTCGGCGACTGCCTGTCCTATGCACTGGC
GATCGATCGACGTGAGCCGCTGCTGTGGAAGGGCAACGACTTTGGGCACACCGGCGTCCAAAGGGCACTGGATCGGCGGT
GA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 8813.12 Da
• Isoelectric Point: 10.3486

>AT28732 WP_003403184.1 NC_020089:707456-707701 [Mycobacterium tuberculosis 7199-99]
MALNIKDPSVHQAVKQIAKITGESQARAVATAVNERLARLRSDDLAARLLAIGHKTASRMSPEAKRLDHDALLYDERGLP
A

Nucleotide

• Length: 246 bp

>AT28732 NC_020089:707456-707701 [Mycobacterium tuberculosis 7199-99]
ATGGCGTTGAACATCAAAGATCCGTCGGTTCACCAGGCGGTCAAGCAGATCGCGAAAATCACCGGCGAATCTCAGGCTCG
GGCGGTGGCGACCGCGGTGAACGAGCGTCTGGCCAGACTGCGCAGCGACGATCTCGCCGCCCGGCTCTTGGCTATCGGCC
ACAAGACCGCGAGCAGGATGAGCCCGGAAGCAAAGCGCCTCGACCACGATGCTCTGCTGTATGACGAGCGAGGGCTGCCG
GCGTGA

Structures

Toxin

• AlphaFold DB: A0A7U4BSD6

Antitoxin

• AlphaFold DB: G0TQ91