Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4059017..4059593 Replicon chromosome
Accession NZ_LR134236
Organism Escherichia coli strain NCTC9008

Toxin (Protein)


Gene name shpA Uniprot ID A0A0E2LC92
Locus tag EL127_RS19930 Protein ID WP_001299718.1
Coordinates 4059306..4059593 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A0E2KXJ8
Locus tag EL127_RS19925 Protein ID WP_000063159.1
Coordinates 4059017..4059319 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EL127_RS19910 4055613..4057763 + 2151 WP_001299714.1 pyruvate/proton symporter BtsT -
EL127_RS19915 4057813..4058016 + 204 WP_000467859.1 YbdD/YjiX family protein -
EL127_RS19920 4058027..4058983 + 957 WP_001299717.1 GTPase -
EL127_RS19925 4059017..4059319 - 303 WP_000063159.1 BrnA antitoxin family protein Antitoxin
EL127_RS19930 4059306..4059593 - 288 WP_001299718.1 BrnT family toxin Toxin
EL127_RS19935 4059790..4060956 + 1167 Protein_3872 restriction endonuclease -
EL127_RS19940 4061020..4062639 + 1620 WP_001029740.1 type I restriction-modification system subunit M -
EL127_RS19945 4062629..4064020 + 1392 Protein_3874 restriction endonuclease subunit S -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11142.60 Da        Isoelectric Point: 7.4697

>T287280 WP_001299718.1 NZ_LR134236:c4059593-4059306 [Escherichia coli]
MPMEFEWDANKAKSNRVKHGIRFEDAVLVFDDPQHLSQQDRIENGEYRWQTIGVVHGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERSRYEHG

Download         Length: 288 bp

>T287280 NZ_LR134236:c4059593-4059306 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGCAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTGGTGTTTGATGATCCACAACATTTGTCACAGCAAGATCGCATAGAAAACGGTGAGTATCGCTGGCAGACTATCGGCG
TGGTTCACGGTATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAGTCGCTATGAGCATGGTTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11536.01 Da        Isoelectric Point: 9.4646

>AT287280 WP_000063159.1 NZ_LR134236:c4059319-4059017 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEEIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT287280 NZ_LR134236:c4059319-4059017 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAATTGAAGGCGCTGGCGAA
TAAATCCGACGAAGAGATTGATTACAGCGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCAGAACGCGGTAAGT
TTTATCGGCCATTGAAAACCCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGCTGAAGCGACCTGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E2LC92

Antitoxin

Source ID Structure
AlphaFold DB A0A0E2KXJ8

References