Detailed information of TA system TA028725

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Rv0299-vapB/-
• Location: 366417..366988
• Replicon: chromosome
• Accession: NC_020089
• Organism: Mycobacterium tuberculosis 7199-99

Toxin (Protein)

• Gene name: Rv0299
• Uniprot ID: -
• Locus tag: MT7199_RS01580
• Protein ID: WP_003401560.1
• Coordinates: 366417..366719 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: O07227
• Locus tag: MT7199_RS01585
• Protein ID: WP_003401563.1
• Coordinates: 366767..366988 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MT7199_RS01560	362189..362992	-	804	WP_003401540.1	hypothetical protein	-
MT7199_RS01565	363002..364399	-	1398	WP_003401544.1	sulfatase	-
MT7199_RS01570	364578..366050	+	1473	WP_015345020.1	PE family protein	-
MT7199_RS01575	366193..366420	+	228	WP_003401555.1	ribbon-helix-helix protein, CopG family	-
MT7199_RS01580	366417..366719	+	303	WP_003401560.1	toxin-antitoxin system toxin	Toxin
MT7199_RS01585	366767..366988	+	222	WP_003401563.1	type II toxin-antitoxin system antitoxin VapB2	Antitoxin
MT7199_RS01590	366985..367410	+	426	WP_003401566.1	PIN domain nuclease	-
MT7199_RS01595	367546..368178	+	633	WP_003401571.1	TetR/AcrR family transcriptional regulator	-
MT7199_RS01600	368175..369083	+	909	WP_003900117.1	SDR family NAD(P)-dependent oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Ribbon-helix-helix protein, CopG (9-37)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 10442.16 Da
• Isoelectric Point: 4.9609

>T28725 WP_003401560.1 NC_020089:366417-366719 [Mycobacterium tuberculosis 7199-99]
MIAPGDIAPRRDSEHELYVAVLSNALHRAADTGRVITCPFIPGRVPEDLLAMVVAVEQPNGTLLPELVQWLHVAALGAPL
GNAGVAALREAASVVTALLC

Nucleotide

• Length: 303 bp

>T28725 NC_020089:366417-366719 [Mycobacterium tuberculosis 7199-99]
TTGATCGCTCCCGGCGACATCGCGCCGCGCCGCGACAGTGAACACGAGCTCTACGTCGCCGTCTTGTCCAACGCGCTCCA
TCGGGCCGCGGACACCGGACGGGTGATCACCTGCCCATTCATTCCGGGCCGGGTCCCCGAGGATCTCTTGGCGATGGTGG
TGGCGGTCGAGCAACCCAACGGCACGCTGCTGCCGGAACTCGTGCAGTGGCTTCATGTTGCCGCGCTCGGTGCGCCACTC
GGCAACGCGGGCGTGGCCGCCCTACGCGAGGCTGCCTCGGTCGTGACAGCTCTGCTCTGTTAG

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8088.31 Da
• Isoelectric Point: 10.7177

>AT28725 WP_003401563.1 NC_020089:366767-366988 [Mycobacterium tuberculosis 7199-99]
MSDVLIRDIPDDVLASLDAIAARLGLSRTEYIRRRLAQDAQTARVTVTAADLRRLRGAVAGLGDPELMRQAWR

Nucleotide

• Length: 222 bp

>AT28725 NC_020089:366767-366988 [Mycobacterium tuberculosis 7199-99]
ATGAGTGATGTACTGATTCGGGACATCCCCGACGACGTGTTAGCAAGCCTTGACGCGATCGCGGCACGCTTGGGCTTGTC
GCGGACCGAATACATCCGTCGGCGTTTAGCCCAGGATGCGCAGACGGCTCGCGTCACCGTGACAGCCGCGGATCTTCGAC
GCCTCAGGGGTGCGGTTGCCGGTCTGGGCGATCCCGAGCTTATGCGTCAGGCGTGGAGGTGA

Structures

Antitoxin

• PDB: 3H87
• AlphaFold DB: A0A829CG48