Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 2882334..2883134 Replicon chromosome
Accession NZ_LR134234
Organism Escherichia coli strain NCTC8623

Toxin (Protein)


Gene name ataT Uniprot ID F4NNI0
Locus tag EL131_RS14305 Protein ID WP_000342449.1
Coordinates 2882334..2882861 (-) Length 176 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID F4NNI1
Locus tag EL131_RS14310 Protein ID WP_001277108.1
Coordinates 2882868..2883134 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EL131_RS14280 2877409..2878176 - 768 WP_000082110.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
EL131_RS14285 2878173..2879450 - 1278 WP_000803798.1 branched chain amino acid ABC transporter permease LivM -
EL131_RS14290 2879447..2880373 - 927 WP_001295111.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
EL131_RS14295 2880421..2881530 - 1110 WP_001352761.1 high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK -
EL131_RS14300 2881954..2882337 + 384 WP_000778781.1 aspartate 1-decarboxylase autocleavage activator PanM -
EL131_RS14305 2882334..2882861 - 528 WP_000342449.1 GNAT family N-acetyltransferase Toxin
EL131_RS14310 2882868..2883134 - 267 WP_001277108.1 DUF1778 domain-containing protein Antitoxin
EL131_RS14315 2883284..2884387 - 1104 WP_001350438.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
EL131_RS14320 2884659..2885513 - 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
EL131_RS14325 2885758..2886816 - 1059 WP_001042003.1 permease-like cell division protein FtsX -
EL131_RS14330 2886809..2887477 - 669 WP_000617723.1 cell division ATP-binding protein FtsE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 176 a.a.        Molecular weight: 19691.70 Da        Isoelectric Point: 7.7457

>T287240 WP_000342449.1 NZ_LR134234:c2882861-2882334 [Escherichia coli]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNKKAHTFYQSLGFIPLVGENENAL
FFPTKSIELLFTQSD

Download         Length: 528 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9910.31 Da        Isoelectric Point: 10.0863

>AT287240 WP_001277108.1 NZ_LR134234:c2883134-2882868 [Escherichia coli]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CLZ4

Antitoxin

Source ID Structure
PDB 6GTS
PDB 6AJN
PDB 6GTQ
PDB 6GTO
PDB 6GTR
PDB 6AJM
AlphaFold DB A0A829CN24

References