Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 2085318..2085901 Replicon chromosome
Accession NZ_LR134234
Organism Escherichia coli strain NCTC8623

Toxin (Protein)


Gene name mazF Uniprot ID U9XFN8
Locus tag EL131_RS10275 Protein ID WP_000254745.1
Coordinates 2085318..2085653 (-) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag EL131_RS10280 Protein ID WP_000581937.1
Coordinates 2085653..2085901 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EL131_RS10260 2081205..2082503 - 1299 WP_000036723.1 phosphopyruvate hydratase -
EL131_RS10265 2082591..2084228 - 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
EL131_RS10270 2084456..2085247 - 792 WP_001071648.1 nucleoside triphosphate pyrophosphohydrolase -
EL131_RS10275 2085318..2085653 - 336 WP_000254745.1 endoribonuclease MazF Toxin
EL131_RS10280 2085653..2085901 - 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
EL131_RS10285 2085979..2088213 - 2235 WP_000226815.1 GTP pyrophosphokinase -
EL131_RS10290 2088261..2089562 - 1302 WP_000046785.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12158.08 Da        Isoelectric Point: 8.4777

>T287237 WP_000254745.1 NZ_LR134234:c2085653-2085318 [Escherichia coli]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPYTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT287237 WP_000581937.1 NZ_LR134234:c2085901-2085653 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYM3

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References