Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 772016..772542 Replicon chromosome
Accession NZ_LR134234
Organism Escherichia coli strain NCTC8623

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag EL131_RS03775 Protein ID WP_000323025.1
Coordinates 772016..772303 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag EL131_RS03780 Protein ID WP_000534858.1
Coordinates 772303..772542 (-) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EL131_RS03730 767040..767255 - 216 WP_000839590.1 phage lysis protein EssD -
EL131_RS24485 767475..767645 + 171 WP_211180497.1 putative zinc-binding protein YnfU -
EL131_RS03735 768009..768224 - 216 WP_000066484.1 cold shock-like protein CspB -
EL131_RS03740 768525..768737 + 213 WP_000087756.1 cold shock-like protein CspF -
EL131_RS03745 768792..768881 + 90 WP_120795389.1 hypothetical protein -
EL131_RS03750 769159..769911 - 753 WP_001047135.1 antitermination protein -
EL131_RS03755 769925..770974 - 1050 WP_001265198.1 DUF968 domain-containing protein -
EL131_RS03760 770976..771254 - 279 WP_012304870.1 hypothetical protein -
EL131_RS03765 771321..771572 - 252 WP_000980994.1 hypothetical protein -
EL131_RS03770 771789..771944 - 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
EL131_RS03775 772016..772303 - 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
EL131_RS03780 772303..772542 - 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
EL131_RS03785 772567..772872 + 306 WP_001326990.1 hypothetical protein -
EL131_RS03790 773075..773407 + 333 WP_001301033.1 protein FlxA -
EL131_RS03795 773844..775157 - 1314 WP_000589005.1 ISNCY family transposase -
EL131_RS24370 775926..776213 - 288 Protein_750 hypothetical protein -
EL131_RS03810 776824..777180 - 357 WP_001310834.1 class I SAM-dependent methyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 736653..785659 49006
- inside Prophage - - 736653..790821 54168


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T287231 WP_000323025.1 NZ_LR134234:c772303-772016 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT287231 WP_000534858.1 NZ_LR134234:c772542-772303 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References