Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 859851..860505 Replicon chromosome
Accession NZ_LR134226
Organism Escherichia coli strain NCTC9088

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag EL094_RS04260 Protein ID WP_000244772.1
Coordinates 860098..860505 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag EL094_RS04255 Protein ID WP_000354046.1
Coordinates 859851..860117 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EL094_RS04230 855139..855882 + 744 WP_000951964.1 SDR family oxidoreductase -
EL094_RS04235 855939..857372 - 1434 WP_001344773.1 6-phospho-beta-glucosidase BglA -
EL094_RS04240 857417..857728 + 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
EL094_RS04245 857892..858551 + 660 WP_000250274.1 hemolysin III family protein -
EL094_RS04250 858628..859608 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
EL094_RS04255 859851..860117 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
EL094_RS04260 860098..860505 + 408 WP_000244772.1 protein YgfX Toxin
EL094_RS04265 860545..861066 - 522 WP_001055874.1 flavodoxin FldB -
EL094_RS04270 861178..862074 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
EL094_RS04275 862099..862809 + 711 WP_000715212.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
EL094_RS04280 862815..864548 + 1734 WP_000813215.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T287148 WP_000244772.1 NZ_LR134226:860098-860505 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT287148 WP_000354046.1 NZ_LR134226:859851-860117 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References