Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4048120..4048696 Replicon chromosome
Accession NZ_LR134220
Organism Escherichia coli strain NCTC11121

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag EL088_RS19890 Protein ID WP_001297643.1
Coordinates 4048409..4048696 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A829G8Z0
Locus tag EL088_RS19885 Protein ID WP_000063156.1
Coordinates 4048120..4048422 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EL088_RS19870 4044716..4046866 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
EL088_RS19875 4046916..4047119 + 204 WP_000467859.1 YbdD/YjiX family protein -
EL088_RS19880 4047130..4048086 + 957 WP_001297640.1 GTPase -
EL088_RS19885 4048120..4048422 - 303 WP_000063156.1 BrnA antitoxin family protein Antitoxin
EL088_RS19890 4048409..4048696 - 288 WP_001297643.1 BrnT family toxin Toxin
EL088_RS19895 4048895..4049592 - 698 Protein_3868 IS1 family transposase -
EL088_RS19900 4049649..4049744 + 96 Protein_3869 dienelactone hydrolase family protein -
EL088_RS19905 4049890..4051122 + 1233 WP_111724804.1 multidrug efflux MFS transporter MdtM -
EL088_RS19910 4051163..4052443 + 1281 WP_097729284.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T287121 WP_001297643.1 NZ_LR134220:c4048696-4048409 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.99 Da        Isoelectric Point: 9.4646

>AT287121 WP_000063156.1 NZ_LR134220:c4048422-4048120 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A829G8Z0

References