Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 3948982..3949558 Replicon chromosome
Accession NZ_LR134216
Organism Escherichia coli strain NCTC10973

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag EL103_RS19120 Protein ID WP_001297643.1
Coordinates 3949271..3949558 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A829G8Z0
Locus tag EL103_RS19115 Protein ID WP_000063156.1
Coordinates 3948982..3949284 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EL103_RS19100 3945578..3947728 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
EL103_RS19105 3947778..3947981 + 204 WP_000467859.1 YbdD/YjiX family protein -
EL103_RS19110 3947992..3948948 + 957 WP_001297640.1 GTPase -
EL103_RS19115 3948982..3949284 - 303 WP_000063156.1 BrnA antitoxin family protein Antitoxin
EL103_RS19120 3949271..3949558 - 288 WP_001297643.1 BrnT family toxin Toxin
EL103_RS19125 3949757..3950454 - 698 Protein_3721 IS1 family transposase -
EL103_RS19130 3950511..3950606 + 96 Protein_3722 dienelactone hydrolase family protein -
EL103_RS19135 3950752..3951984 + 1233 WP_001339512.1 multidrug efflux MFS transporter MdtM -
EL103_RS19140 3952025..3953305 + 1281 WP_001037380.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 3949757..3949966 209


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T287102 WP_001297643.1 NZ_LR134216:c3949558-3949271 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.99 Da        Isoelectric Point: 9.4646

>AT287102 WP_000063156.1 NZ_LR134216:c3949284-3948982 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A829G8Z0

References