Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PemK-MazE
Location 493754..494298 Replicon plasmid pRtrCIAT899b
Accession NC_020061
Organism Rhizobium tropici CIAT 899

Toxin (Protein)


Gene name mazF Uniprot ID N6V6D9
Locus tag RTCIAT899_RS21955 Protein ID WP_004119734.1
Coordinates 493975..494298 (+) Length 108 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID N6V0T1
Locus tag RTCIAT899_RS21950 Protein ID WP_004119731.1
Coordinates 493754..493978 (+) Length 75 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RTCIAT899_RS21935 489251..489508 + 258 WP_004119722.1 hypothetical protein -
RTCIAT899_RS33275 489821..489998 + 178 Protein_463 IS5/IS1182 family transposase -
RTCIAT899_RS21940 490953..492203 + 1251 WP_004119727.1 MFS transporter -
RTCIAT899_RS21945 492452..493357 + 906 WP_004119729.1 hypothetical protein -
RTCIAT899_RS21950 493754..493978 + 225 WP_004119731.1 antitoxin MazE family protein Antitoxin
RTCIAT899_RS21955 493975..494298 + 324 WP_004119734.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
RTCIAT899_RS31725 494373..494759 - 387 Protein_468 hypothetical protein -
RTCIAT899_RS32880 494838..495473 - 636 WP_051043309.1 aminotransferase class I/II-fold pyridoxal phosphate-dependent enzyme -
RTCIAT899_RS33280 496089..496298 - 210 WP_135488292.1 hypothetical protein -
RTCIAT899_RS21970 496449..496783 - 335 Protein_471 type II toxin-antitoxin system RelE/ParE family toxin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..549467 549467


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(2-103)

Antitoxin

Antitoxin MazE-like

(6-70)


Sequences

Toxin        


Download         Length: 108 a.a.        Molecular weight: 11356.28 Da        Isoelectric Point: 10.7342

>T28708 WP_004119734.1 NC_020061:493975-494298 [Rhizobium tropici CIAT 899]
VRRGDIVTVAISGDFGKPRPALIIQADQFDATGTITVLLISSTLVDAPLIRPTIQPTVQSGLRKASQVMVDKAMSVKRDR
IGTTIGRLEDDALLAVTRSLAVFLGVA

Download         Length: 324 bp

>T28708 NC_020061:493975-494298 [Rhizobium tropici CIAT 899]
GTGAGGCGCGGTGACATTGTGACCGTCGCAATCAGCGGTGATTTCGGCAAGCCGCGTCCAGCACTAATCATCCAGGCCGA
TCAGTTTGATGCAACTGGAACGATCACTGTCCTGCTGATCTCCAGCACACTGGTCGACGCGCCGCTCATTCGACCAACCA
TTCAGCCGACAGTGCAAAGCGGATTGCGAAAAGCCTCGCAAGTGATGGTGGACAAAGCCATGTCCGTGAAACGGGATAGA
ATTGGCACAACGATCGGTCGCCTGGAAGATGATGCGCTCCTGGCGGTCACAAGATCGCTCGCCGTTTTTTTAGGTGTTGC
TTAA

Antitoxin


Download         Length: 75 a.a.        Molecular weight: 8147.02 Da        Isoelectric Point: 4.3939

>AT28708 WP_004119731.1 NC_020061:493754-493978 [Rhizobium tropici CIAT 899]
MASSVNERVQKRRETLRAAGLRPVQIWVPDTRRPGFAAECHRQSVQVALADAADPSLQDFLDAALDDLDDEGEA

Download         Length: 225 bp

>AT28708 NC_020061:493754-493978 [Rhizobium tropici CIAT 899]
ATGGCAAGCTCGGTCAACGAACGAGTTCAAAAGCGCCGTGAGACGTTGCGCGCCGCTGGGCTGCGTCCTGTGCAAATATG
GGTTCCCGATACTCGTCGCCCTGGTTTTGCCGCCGAATGCCATCGTCAGTCCGTTCAGGTAGCCCTTGCCGATGCCGCCG
ATCCCAGTCTGCAAGACTTCCTTGACGCCGCTCTCGATGACCTGGACGACGAGGGTGAAGCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5F1S1T4

Antitoxin

Source ID Structure
AlphaFold DB A0A7W8XV61

References