Detailed information of TA system TA028705

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicB-HicA
• Location: 55951..56405
• Replicon: chromosome
• Accession: NC_019977
• Organism: Methanomethylovorans hollandica DSM 15978

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: L0KWL1
• Locus tag: METHO_RS00265
• Protein ID: WP_015323518.1
• Coordinates: 56172..56405 (+)
• Length: 78 a.a.

Antitoxin (Protein)

• Gene name: hicA
• Uniprot ID: L0KWC1
• Locus tag: METHO_RS00260
• Protein ID: WP_015323517.1
• Coordinates: 55951..56175 (+)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
METHO_RS00230 (Metho_0045)	51302..53110	+	1809	WP_015323511.1	hypothetical protein	-
METHO_RS00235 (Metho_0046)	53162..53494	+	333	WP_015323512.1	hypothetical protein	-
METHO_RS00240 (Metho_0047)	53600..53845	-	246	WP_015323513.1	type II toxin-antitoxin system HicA family toxin	-
METHO_RS00245 (Metho_0048)	53842..54060	-	219	WP_048830935.1	type II toxin-antitoxin system HicB family antitoxin	-
METHO_RS00250 (Metho_0049)	54380..55627	+	1248	WP_015323515.1	DUF2254 family protein	-
METHO_RS00260 (Metho_0051)	55951..56175	+	225	WP_015323517.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
METHO_RS00265 (Metho_0052)	56172..56405	+	234	WP_015323518.1	type II toxin-antitoxin system HicA family toxin	Toxin
METHO_RS00270 (Metho_0053)	56486..57184	-	699	WP_015323519.1	fibrillarin-like rRNA/tRNA 2'-O-methyltransferase	-
METHO_RS00275 (Metho_0054)	57181..58281	-	1101	WP_015323520.1	NOP5/NOP56 family protein	-
METHO_RS00280 (Metho_0055)	58283..59251	-	969	WP_015323521.1	beta-ribofuranosylaminobenzene 5'-phosphate synthase	-
METHO_RS00285 (Metho_0056)	59323..59988	-	666	WP_172635176.1	DUF2953 domain-containing protein	-
METHO_RS00290 (Metho_0057)	60100..60522	-	423	WP_015323523.1	spore germination protein GerW family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (10-64)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (5-69)

Sequences

Toxin

Protein

• Length: 78 a.a.
• Molecular weight: 8955.40 Da
• Isoelectric Point: 9.5917

>T28705 WP_015323518.1 NC_019977:56172-56405 [Methanomethylovorans hollandica DSM 15978]
MSRLPLVDARTMEKILYNLGFEKVRQKGSHAFYRHADGRTTTIPFHSSKELARPLIRVILNEINLTIEEYGELLKDI

Nucleotide

• Length: 234 bp

>T28705 NC_019977:56172-56405 [Methanomethylovorans hollandica DSM 15978]
ATGAGCAGATTGCCATTGGTCGATGCCCGAACAATGGAAAAGATACTGTATAATCTTGGATTTGAAAAGGTCAGACAAAA
AGGCAGTCATGCATTTTACAGGCATGCCGATGGTAGAACGACCACAATACCCTTTCATTCAAGCAAAGAACTTGCAAGGC
CTCTCATAAGAGTCATTCTGAATGAGATCAACCTCACTATTGAAGAATATGGCGAATTATTGAAGGATATTTAG

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8222.44 Da
• Isoelectric Point: 3.9213

>AT28705 WP_015323517.1 NC_019977:55951-56175 [Methanomethylovorans hollandica DSM 15978]
MISKFTAYIEKDVETGCYVAIVPGIPGAHTQAETLDELQVRLKEVIELCLEEMSPEDKESIPEFVGLQQIEVAV

Nucleotide

• Length: 225 bp

>AT28705 NC_019977:55951-56175 [Methanomethylovorans hollandica DSM 15978]
ATGATAAGCAAATTCACTGCATATATAGAGAAAGATGTGGAAACCGGCTGCTATGTTGCTATTGTCCCGGGAATTCCCGG
AGCTCATACACAAGCTGAAACACTGGATGAATTGCAGGTTCGGTTAAAAGAAGTGATCGAGCTGTGCCTTGAAGAAATGA
GCCCTGAAGATAAAGAATCGATCCCCGAGTTCGTAGGCCTTCAGCAAATTGAGGTTGCTGTATGA

Structures

Toxin

• AlphaFold DB: L0KWL1

Antitoxin

• AlphaFold DB: L0KWC1