Detailed information of TA system TA028704

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN-PHD
• Location: 4654965..4655563
• Replicon: chromosome
• Accession: NC_019973
• Organism: Mesorhizobium australicum WSM2073

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: MESAU_RS22475
• Protein ID: WP_015318320.1
• Coordinates: 4655186..4655563 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: L0KND7
• Locus tag: MESAU_RS22470
• Protein ID: WP_015318319.1
• Coordinates: 4654965..4655189 (+)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MESAU_RS22440	4650044..4650856	+	813	WP_015318313.1	metal ABC transporter ATP-binding protein	-
MESAU_RS22445	4650856..4651731	+	876	WP_015318314.1	metal ABC transporter permease	-
MESAU_RS22450	4651763..4652698	+	936	WP_015318315.1	metal ABC transporter substrate-binding protein	-
MESAU_RS22455	4652848..4653186	+	339	WP_015318316.1	TIGR01244 family phosphatase	-
MESAU_RS22460	4653183..4653650	-	468	WP_041163864.1	Lrp/AsnC family transcriptional regulator	-
MESAU_RS22465	4653786..4654898	+	1113	WP_015318318.1	4-hydroxyphenylpyruvate dioxygenase	-
MESAU_RS22470	4654965..4655189	+	225	WP_015318319.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
MESAU_RS22475	4655186..4655563	+	378	WP_015318320.1	type II toxin-antitoxin system VapC family toxin	Toxin
MESAU_RS22480	4655568..4656104	-	537	WP_015318321.1	DinB family protein	-
MESAU_RS22485	4656126..4657139	-	1014	WP_015318322.1	fumarylacetoacetate hydrolase family protein	-
MESAU_RS22490	4657136..4658023	-	888	WP_015318323.1	MBL fold metallo-hydrolase	-
MESAU_RS22495	4658035..4659348	-	1314	WP_015318324.1	homogentisate 1,2-dioxygenase	-
MESAU_RS22500	4659436..4659873	+	438	WP_015318325.1	MarR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (8-121)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (3-40)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13855.92 Da
• Isoelectric Point: 5.9626

>T28704 WP_015318320.1 NC_019973:4655186-4655563 [Mesorhizobium australicum WSM2073]
MTAGSFLADTQIILWSIKDDPRLSEEHRAVLASDATVYASAASVWEIAIKRSIGKLDVPDDLPALLPRMQFQPLTITFQH
AHVVGRLPRHHGDPFDRLLIAQAQVENLTILTADKQFALYDVALA

Nucleotide

• Length: 378 bp

>T28704 NC_019973:4655186-4655563 [Mesorhizobium australicum WSM2073]
ATGACTGCCGGCTCCTTTCTTGCCGATACGCAAATTATCCTTTGGTCTATTAAGGACGACCCTCGACTCAGCGAGGAGCA
CCGGGCTGTCCTTGCATCGGACGCGACCGTTTACGCCAGCGCGGCAAGCGTTTGGGAAATCGCGATCAAGCGATCGATCG
GCAAACTCGACGTTCCGGACGACCTGCCGGCGTTACTCCCGAGAATGCAATTCCAGCCGCTGACCATCACGTTTCAGCAT
GCGCATGTTGTCGGCCGCCTTCCCCGCCACCATGGCGATCCGTTCGACCGGCTGCTGATCGCCCAAGCGCAGGTCGAGAA
TCTCACCATCCTGACAGCGGACAAGCAATTTGCTCTCTATGACGTCGCGCTGGCATGA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8152.45 Da
• Isoelectric Point: 5.0790

>AT28704 WP_015318319.1 NC_019973:4654965-4655189 [Mesorhizobium australicum WSM2073]
MNVSIAEAKAKLSELISRAQAGEEIVLTRHGKIAARLVPPLGENLLPRIGALKGKIWIADDFDELGPEWDEYVK

Nucleotide

• Length: 225 bp

>AT28704 NC_019973:4654965-4655189 [Mesorhizobium australicum WSM2073]
ATGAATGTTTCCATTGCAGAAGCCAAGGCAAAACTGTCGGAACTGATCAGCCGTGCGCAAGCTGGCGAAGAGATCGTGCT
TACACGTCACGGCAAGATCGCGGCTCGGCTGGTGCCACCGCTCGGCGAAAATCTCCTGCCCCGTATTGGCGCCTTAAAGG
GCAAGATTTGGATAGCGGACGATTTTGACGAACTCGGTCCCGAATGGGACGAATACGTCAAATGA

Structures

Antitoxin

• AlphaFold DB: A0A503E6P1