Detailed information of TA system TA028701

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TacT2-ataR/DUF1778(antitoxin)
• Location: 3373076..3373782
• Replicon: chromosome
• Accession: NC_019973
• Organism: Mesorhizobium australicum WSM2073

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: L0KKU9
• Locus tag: MESAU_RS16230
• Protein ID: WP_015317124.1
• Coordinates: 3373297..3373782 (+)
• Length: 162 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: -
• Locus tag: MESAU_RS16225
• Protein ID: WP_085986600.1
• Coordinates: 3373076..3373297 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MESAU_RS16200	3368392..3369378	+	987	WP_015317118.1	ABC transporter permease	-
MESAU_RS16205	3369423..3369929	+	507	WP_167331156.1	gluconokinase	-
MESAU_RS16210	3369924..3371072	-	1149	WP_015317120.1	hypothetical protein	-
MESAU_RS16215	3371083..3371532	-	450	WP_023769580.1	Rrf2 family transcriptional regulator	-
MESAU_RS16220	3371784..3372770	-	987	WP_015317123.1	sulfate ABC transporter substrate-binding protein	-
MESAU_RS16225	3373076..3373297	+	222	WP_085986600.1	DUF1778 domain-containing protein	Antitoxin
MESAU_RS16230	3373297..3373782	+	486	WP_015317124.1	GNAT family N-acetyltransferase	Toxin
MESAU_RS16235	3373827..3374867	-	1041	WP_015317125.1	sulfate/molybdate ABC transporter ATP-binding protein	-
MESAU_RS16240	3374880..3375764	-	885	WP_015317126.1	sulfate ABC transporter permease subunit CysW	-
MESAU_RS16245	3375757..3376608	-	852	WP_015317127.1	sulfate ABC transporter permease subunit CysT	-
MESAU_RS16250	3376631..3377659	-	1029	WP_015317128.1	sulfate ABC transporter substrate-binding protein	-
MESAU_RS16255	3377882..3378613	-	732	WP_174361969.1	M15 family metallopeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (41-142)

Antitoxin

• Vibrio phage ICP1, Orf50 (4-57)

Sequences

Toxin

Protein

• Length: 162 a.a.
• Molecular weight: 17139.72 Da
• Isoelectric Point: 9.5219

>T28701 WP_015317124.1 NC_019973:3373297-3373782 [Mesorhizobium australicum WSM2073]
MLSAPTPLAENHDLELFQSGTESLDQWLRRRARANQVSGASRTYVVAEGTRVVGYYCLSSGGLDLAEAPGIVRRNMPDPI
PMVVLGRLAVDTGWQGKGPGAALLQDAVLRAGQAATILGIRGIFVHAISDEAKAFYERHGFAASPKNPMTLVLSLKGLVK
G

Nucleotide

• Length: 486 bp

>T28701 NC_019973:3373297-3373782 [Mesorhizobium australicum WSM2073]
ATGCTGTCGGCGCCCACGCCGCTGGCCGAGAACCACGATCTCGAACTTTTCCAAAGCGGAACGGAAAGTCTCGACCAATG
GCTTCGCCGCCGCGCACGGGCCAACCAGGTCAGTGGCGCATCTCGTACTTATGTGGTCGCGGAAGGCACACGCGTTGTCG
GCTACTACTGCCTGTCTTCGGGCGGGCTTGACCTGGCGGAAGCGCCCGGCATTGTTCGGCGCAACATGCCGGATCCCATT
CCAATGGTCGTGCTCGGGCGTCTCGCGGTGGACACAGGCTGGCAAGGGAAGGGACCGGGTGCAGCGCTTCTGCAAGATGC
CGTCCTGCGCGCCGGTCAAGCCGCGACGATCCTCGGGATACGCGGAATTTTTGTTCATGCGATATCGGACGAAGCGAAAG
CCTTTTATGAGCGCCATGGCTTTGCGGCTTCCCCGAAAAATCCCATGACATTGGTGCTGTCGTTGAAAGGCCTGGTGAAA
GGGTAG

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8408.62 Da
• Isoelectric Point: 5.7879

>AT28701 WP_085986600.1 NC_019973:3373076-3373297 [Mesorhizobium australicum WSM2073]
MRILIDRAAKLRGKTRSDFMIDAAYRAAEDTLLDQTLIKVDAQSYRHYLDILDEPPSGEGFARLMNVPKPWQD

Nucleotide

• Length: 222 bp

>AT28701 NC_019973:3373076-3373297 [Mesorhizobium australicum WSM2073]
ATGCGCATACTGATTGACCGCGCCGCAAAGCTACGCGGGAAAACTCGTTCCGATTTCATGATCGATGCCGCCTACCGCGC
GGCCGAGGATACGCTCCTCGACCAAACACTGATCAAGGTAGATGCCCAGAGTTACCGGCACTATCTCGACATCCTCGATG
AACCGCCAAGCGGGGAAGGTTTTGCGCGTCTTATGAACGTGCCCAAGCCATGGCAGGACTGA

Structures

Toxin

• AlphaFold DB: L0KKU9